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Therapeutic applications of ribozymes and riboswitches: methods and protocols
2014, Humana Press
in English
1627037292 9781627037297
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Book Details
Table of Contents
Identification of regulatory RNA in bacterial genomes by genome-scale mapping of transcription start sites --
Screening inhibitory potential of anti-HIV RT RNA aptamers --
Design and evaluation of clinically relevant SOFA-HDV ribozymes targeting HIV RNA --
Directing RNase P-mediated cleavage of target mRNAs by engineered external guide sequences in cultured cells --
Design and analysis of hammerhead ribozyme activity against an artificial gene target --
Knockdown strategies for the study of proprotein convertases and proliferation in prostate cancer cells --
Use of tumor-targeting trans-splicing ribozyme for cancer treatment --
Characterization of hairpin ribozyme reactions --
Finding instances of riboswitches and ribozymes by homology search of structured RNA with infernal --
Structure-based virtual screening for the identification of RNA- binding ligands --
Probing riboswitch binding sites with molecular docking, focused libraries, and in-line probing assays --
Discovery of small molecule modifiers of micrornas for the treatment of HCV infection --
Bacterial flavin mononucleotide riboswitches as targets for flavin analogs --
Construction and application of riboswitch-based sensors that detect metabolites within bacterial cells --
Screening assays to identify artificial glmS ribozyme activators --
Analysis of riboswitch structure and ligand binding using small-angle X-ray scattering (SAXS) --
Use of SHAPE to select 2AP substitution sites for RNA-ligand interactions and dynamics studies --
Cell internalization SELEX: In vitro selection for molecules that internalize into cells --
DNA electronic switches based on analyte-responsive aptamers.
Edition Notes
Includes bibliographical references and index.
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Feedback?February 26, 2022 | Edited by ImportBot | import existing book |
November 12, 2020 | Created by MARC Bot | import new book |