Pt. I. Basic Concepts. Recent advances in non-viral gene delivery / Christine C. Conwell and Leaf Huang. Barriers to gene delivery using synthetic vectors / Martin L. Read, Ann Logan, and Leonard W. Seymour. Pharmacokinetics of plasmid DNA-based non-viral gene medicine / Makiya Nishikawa, Yoshinobu Takakura, and Mitsuru Hashida. What role can chemistry play in cationic liposome-based gene therapy research today? / Kostas Kostarelos and Andrew D. Miller. Lipoplex structures and their distinct cellular pathways / Kai Ewert ... [et al.] Diffusible-PEG-lipid stabilized plasmid lipid particles / Ian MacLachlan and Pieter Cullis. Toxicity of cationic lipid-DNA complexes / Nelson S. Yew and Ronald K. Scheule. Polyethylenimine (PEI) / Barbara Demeneix and Jean-Paul Behr. Pluronic block copolymers for gene delivery / Alexander Kabanov, Jian Zhu, and Valery Alakhov. Terplex gene delivery system / Sung Wan Kim. Design of polyphosphoester-DNA nanoparticles for non-viral gene delivery / Hai-Quan Mao and Kam W. Leong. Development of HVJ envelope vector and its application to gene therapy / Yasufumi Kaneda, Seiji Yamamoto, and Toshihiro Nakajima. Targeting of polyplexes: toward synthetic virus vector systems / Ernst Wagner, Carsten Culmsee, and Sabine Boeckle

Pt. II. Naked DNA, oligonucleotide and physical methods. The mechanism of naked DNA uptake and expression / Jon A. Wolff and Vladimir Budker. Targeted delivery of therapeutic oligonucleotides to pulmonary circulation / Annette Wilson ... [et al.]. Naked DNA for liver gene transfer / Feng Liu and Pradeep Tyagi. Hydrodynamic delivery / Mohammed S. Al-Dosari, Joseph E. Knapp, and Dexi Liu. Electric pulse-mediated gene delivery to various animal tissues / Lluis M. Mir ... [et al.] In vivo application of RNA interference: from functional genomics to therapeutics / Patrick Y. Lu, Frank Xie, and Martin C. Woodle. A novel gene expression system: non-viral gene transfer for hemophilia as model systems / Carol H. Miao. Site-specific integration with [phi]C31 integrase for prolonged expression of therapeutic genes / Daniel S. Ginsburg and Michele P. Calos. Sleeping Beauty transposon-mediated gene therapy for prolonged expression / Perry B. Hackett ... [et al.] Cancer-specific gene therapy / Hui-Wen Lo, Chi-Ping Day, and Mien-Chie Hung. DNA vaccine / Zhengrong Cui. Airway gene therapy / Jane C. Davies and Eric W.F.W. Alton. Non-viral vector as vaccine carrier / Weihsu Claire Chen and Leaf Huang. Non-viral vectors for gene therapy: clinical trials in cardiovascular disease / Pinak B. Shah and Douglas W. Losordo.