An edition of Rna Protein Interaction Protocols
(2008)
RNA-protein interaction protocols.
2nd ed. / edited by Ren-Jang Lin.
by Lin Ren-jang
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Due to the vital biological importance of RNA and proteins functioning together within a cell, a protocol volume describing experimental procedures to study their interactions should find a home in many laboratories. This second edition complements and expands upon the popular first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. The contributors explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.
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| Edition | Availability |
|---|---|
1
Rna Protein Interaction Protocols: Preliminary Entry 2139 (Methods in Molecular Biology)
November 2008, Humana Press
Hardcover
in English
- 2 Revised edition
1588294196 9781588294197
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2
RNA-protein interaction protocols.
2008, Humana, Springer [distributor]
in English
- 2nd ed. / edited by Ren-Jang Lin.
1588294196 9781588294197
|
aaaa
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Book Details
Table of Contents
1. Isolation of a sequence-specific RNA binding protein, polypyrimidine tract binding protein using RNA affinity chromatography / Shalini Sharma
2. An affinity oligonucleotide displacement strategy to purify ribonucleoprotein complexes applied to human telomerase /Isabel Kurth, Gaël Cristofari, Joachim Lingner
3. RNA affinity tags for the rapid purification and investigation of RNAs and RNA-protein complexes / Scott C. Walker ... [et al.]
4. Assembly and glycerol gradient isolation of yeast spliceosomes containing transcribed or synthetic U6 SnRNA / Ken Dery, Shyue-Lee Yean, Ren-Jang Lin
5. Purification of ribonucleoproteins using peptide-elutable antibodies and other affinity techniques / Scott Stevens
6. CLIP: cross-linking and immunoprecipitation of in vivo RNA targets of RNA binding proteins / Kirk B Jensen, Robert B. Darnell
7. Quantitative analysis of protein-RNA interactions by gel mobility shift / Sean P. Ryder, Michael I. Recht, James R. Williamson
8. Monitoring assembly of ribonucleoprotein complexes by isothermal titration calorimetry / Michael I. Recht, Sean P. Ryder, James R. Williamson
9. Characterization of RNA-protein interactions by phosphorothioate footprinting and its applications to the ribosome / A. Ozlem Tastan Bishop, ... [et al.]
10. In vivo analysis of ribonucleoprotein complexes using nucleotide analog interference mapping / Lara Weinstein Szewczak
11. T7 RNA polymerase mediated incorporation of 8-N3AMP into RNA for studying protein-RNA interactions / Rajesh Gaur
12. A simple crosslinking method, CLAMP, to map the sites of RNA contacting domains within a protein / Hiren Banerjee, Ravinder Singh
13. Proteins specifically modified with a chemical nuclease as probes of RNA-protein interaction / Oliver A. Ken, Andrew M. MacMillan
14. RNA-protein crosslink mapping using TEV protease / Ian A. Turner, ... [et al.]
15 Structural analysis of protein-RNA interactions with mass spectrometry / Mamuka Kvaratskhelia, Stuart F.J. Le Grice
16. Analysing RNA-protein cross-linking sites in unlabeled ribonucleoprotein complexes by mass spectrometry / Henning Urlaub, Eva Kühn-Hölsken, Reinhard Lührmann
17. In vitro selection of random RNA fragments to identify protein binding sites within large RNAs / Ulrich Stelzl, Knud H. Nierhaus
18. Immunoprecipitation analysis to study RNA-protein interactions in xenopus oocytes / Naoto Mabuchi, Kaoru Masuyama, Mutsuhito Ohno
19. Mapping the regions of RNase P catalytic RNA that are potentially in close contact with its protein cofactor / Phong Trang, Fenyong Liu
20. Quantification of microRNAs, splicing isoforms, and homologous mRNAs with the invader assay / Peggy S. Eis, Mariano Garcia-Blanco
21. Analysis of RNA structure and RNA-protein interactions in mammalian cells by use of terminal transferase-dependent PCR / Hsiu-Hua Chen, Jeanne LeBon, Arthur D. Riggs
22. Duplex unwinding and RNP remodeling with RNA helicases / Eckhard Jankowsky, Margaret E. Fairman
23. Preparation of efficient splicing extracts from whole cells, nuclei, and cytoplasmic fractions / Naoyuki Kataoka, Gideon Dreyfuss
24. Designing and utilization of siRNAs targeting RNA-binding proteins / Dong-Ho Kim, Mark Behlke, John J. Rossi
25. The use of saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins / Jane E. Jackman, Eric M. Phizicky.
Edition Notes
Previous ed.: 1999.
Includes bibliographical references and index.
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