An edition of RNA folding (2014)

RNA folding

methods and protocols

RNA folding
Christina Waldsich, Christina ...
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Last edited by MARC Bot
October 6, 2024 | History
An edition of RNA folding (2014)

RNA folding

methods and protocols

Focusing on in vitro and intracellular RNA structure formation, RNA Folding: Methods and Protocols provides a comprehensive collection of experimental protocols which are suitable to dissect RNA folding pathways and to characterize the structure of RNA folding intermediates at nucleotide or even atomic resolution. The presented techniques include powerful tools with a long tradition in RNA research as well as more advanced, novel methods, thus the methods span multiple disciplines, including molecular biology, biochemistry, biophysics, and computational biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, RNA Folding: Methods and Protocols serves as a vital reference for researchers attempting to gain insights into the secrets of this astounding macromolecule.

Publish Date
Publisher
Humana Press
Language
English
Pages
340

Buy this book

Edition Availability
Cover of: RNA Folding
RNA Folding: Methods and Protocols
2016, Humana Press
in English
Cover of: RNA folding
RNA folding: methods and protocols
2014, Humana Press
in English

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Book Details


Table of Contents

Predicting RNA Structure: Advances and Limitations
Cis-Acting 5' Hammerhead Optimization for In Vitro Transcription of Highly Structured RNAs
RNA Structural Analysis by Enzymatic Digestion
A Mutate-and-Map Protocol for Inferring Base Pairs in Structured RNA
Mapping RNA Structure In Vitro Using Nucleobase-Specific Probes
Massively Parallel RNA Chemical Mapping with a Reduced Bias MAP-Seq Protocol
Probing RNA Folding by Hydroxyl Radical Footprinting
Monitoring Global Structural Changes and Specific Metal Binding Sites in RNA by In-Line Probing and Tb(III) Cleavage
Chemical Probing of RNA in Living Cells
A Chemogenetic Approach to Study the Structural Basis of Protein-Facilitated RNA Folding
Applying UV Crosslinking to Study RNA-Protein Interactions in Multicomponent Ribonucleoprotein Complexes
The Kinetics of Ribozyme Cleavage: A Tool to Analyze RNA Folding as a Function of Catalysis
RNA Catalytic Activity as a Probe of Chaperone-Mediated RNA Folding
Evaluation of RNA Chaperone Activity In Vivo and In Vitro Using Misfolded Group I Ribozymes
RNA Conformational Changes Analyzed by Comparative Gel Electrophoresis
Detecting RNA Tertiary Folding by Sedimentation Velocity Analytical Ultracentrifugation
RNA Folding Dynamics Using Laser Assisted Single-Molecule Refolding
RNA Refolding Studied by Light-Coupled NMR Spectroscopy
Exploring RNA Oligomerization and Ligand Binding by Fluorescence Correlation Spectroscopy and Small Angle X-Ray Scattering.

Edition Notes

Includes bibliographical references and index.

Published in
New York
Series
Methods in molecular biology -- 1086, Springer protocols, Methods in molecular biology (Clifton, N.J.) -- v. 1086., Springer protocols (Series)
Copyright Date
2014

Classifications

Dewey Decimal Class
572.8/8
Library of Congress
QP623 .R57265 2014, QH506 .M45 v.1086, RB155-155.8QH431QD43, QP623

The Physical Object

Pagination
x, 340 pages
Number of pages
340

Edition Identifiers

Open Library
OL31014534M
ISBN 10
1627036660
ISBN 13
9781627036665, 9781627036672
LCCN
2013949278
OCLC/WorldCat
857276876, 862940963

Work Identifiers

Work ID
OL23177553W

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October 6, 2024 Edited by MARC Bot import existing book
September 13, 2021 Edited by ImportBot import existing book
November 12, 2020 Created by MARC Bot import new book