An edition of Simulations of molecules and processes in the synapse (2002)

Simulations of molecules and processes in the synapse

A dissertation submitted in partial satisfaction of the requirements for the degree Doctor of Philosophy in Chemistry

by

  • 0 Ratings
  • 0 Want to read
  • 0 Currently reading
  • 0 Have read
Simulations of molecules and processes in the ...
Not in Library

My Reading Lists:

Create a new list

Check-In

×Close
Add an optional check-in date. Check-in dates are used to track yearly reading goals.
Today
Notes
  • 0 Ratings
  • 0 Want to read
  • 0 Currently reading
  • 0 Have read
Notes

Check nearby libraries

Buy this book

Last edited by Kaihsu Tai
August 6, 2015 | History
Edit
An edition of Simulations of molecules and processes in the synapse (2002)

Simulations of molecules and processes in the synapse

A dissertation submitted in partial satisfaction of the requirements for the degree Doctor of Philosophy in Chemistry

by

  • 0 Ratings
  • 0 Want to read
  • 0 Currently reading
  • 0 Have read

Abstract

Two nanosecond-timescale molecular dynamics simulations of acetylcholinesterase (EC 3.1.1.7) were analyzed: one unliganded, the other complexed with the snake-venom toxin fasciculin 2. These simulation trajectories revealed complex fluctuation of the active site gorge. In both simulations, we observe a two-peaked probability distribution of the gorge width. Comparing the gorge width with the principal components of motion showed that collective motions of many scales contribute to the opening behavior of the gorge. Covariance and correlation results, as displayed in the novel “porcupine plots”, identified the motions of the protein backbone as the gorge opens.

Fasciculin 2 binds to the gorge entrance of acetylcholinesterase with excellent complementarity and many polar and hydrophobic interactions; it appears to sterically block access of ligands to the gorge. When fasciculin is present, the gorge width distribution is altered such that the gorge is more likely to be narrow. Moreover, there are large increases in the opening of alternative passages, namely the side door and the back door. Finally, the catalytic triad arrangement in the acetylcholinesterase active site is disrupted with fasciculin bound. These data support that, in addition to the steric obstruction seen in the crystal structure, fasciculin may inhibit acetylcholinesterase by combined allosteric and dynamical means.

On a larger scale, the general infrastructure for solving the time-dependent diffusion equation using the finite element method has been implemented. Simulations of synaptic transmission were performed using simplified rectilinear models of the neuromuscular junction to demonstrate the effects of synaptic geometries and reactivity parameters. Observations from models representing synapses in fast- and slow-twitch muscles follow the trends of experimental data. One of our models explains the effects of geometry in muscular dystrophy; another demonstrates the capability of our infrastructure to simulate complicated realistic models based on electron microscopy data.

Publish Date
Publisher
University of California, San Diego
Language
English

Check nearby libraries

Buy this book

Subjects
acetylcholinesterase, fasciculin, finite element method, molecular dynamics, neuromuscular junction

Showing 1 featured edition. View all 1 editions?

Edition Availability
Cover of: Simulations of molecules and processes in the synapse
Simulations of molecules and processes in the synapse: A dissertation submitted in partial satisfaction of the requirements for the degree Doctor of Philosophy in Chemistry
2002, University of California, San Diego
in English
  • Not in Library
Libraries near you: WorldCat

Add another edition?

Book Details

Table of Contents

Signatures of approval . . . . . . . . . . . . . . . . . . . . . . . . . . . . . iii
Dedication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . iv
Epigraph . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Table of contents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vi
List of figures and tables . . . . . . . . . . . . . . . . . . . . . . . . . . . . viii
Acknowledgments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi
Vita, publications, and field of study . . . . . . . . . . . . . . . . . . . . . xii
Abstract . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii
I Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
A. The synapse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
B. Molecular dynamics . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
C. Finite element method . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
D. Acknowledgments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
II Analysis of a 10 ns molecular dynamics simulation of mouse acetylcholinesterase
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
A. Abstract . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
B. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
C. Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
D. Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
E. Discussion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
F. Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
G. Acknowledgments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
III Mechanism of acetylcholinesterase inhibition by fasciculin: 5 ns molecular
dynamics simulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
A. Abstract . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
B. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
C. Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
D. Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
E. Discussion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
F. Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
G. Acknowledgments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
IV Finite element simulations of acetylcholine diffusion in neuromuscular junctions
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
A. Abstract . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
B. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
C. The neuromuscular junction . . . . . . . . . . . . . . . . . . . . . . . . 71
D. Mathematical setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
E. Neuromuscular junction models . . . . . . . . . . . . . . . . . . . . . . 76
F. Discussion and conclusions . . . . . . . . . . . . . . . . . . . . . . . . 88
G. Acknowledgments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
Bibliography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
Colophon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96

Edition Notes

Published in
San Diego, USA

The Physical Object

Pagination
xiv, 96p.

ID Numbers

Open Library
OL25759514M
ISBN 10
049390932X

Community Reviews (0)

Feedback?
No community reviews have been submitted for this work.

Lists

This work does not appear on any lists.

History

Download catalog record: RDF / JSON
August 6, 2015 Edited by Kaihsu Tai Added new cover
August 6, 2015 Edited by Kaihsu Tai add subtitle and keywords
August 6, 2015 Edited by Kaihsu Tai add abstract
August 6, 2015 Edited by Kaihsu Tai Edited without comment.
August 6, 2015 Created by Kaihsu Tai Added new book.