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Antibody phage display 1 edition

Antibody phage display
Robert Aitken

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Antibody phage display
methods and protocols
2nd ed. / edited by Robert Aitken.

Published 2009 by Humana Press in Dordrecht, New York .
Written in English.

Table of Contents

Antibody phage display: overview of a powerful technology that has quickly translated to the clinic / B. Kotlan and M.C. Glassy
Design and construction of synthetic phage-displayed Fab libraries / J. Bostrom and G. Fuh
Affinity isolation of antigen-specific circulating B cells for generation of phage display-derived human monoclonal antibodies / H.J. Ditzel
Construction of phage antibody repertoires from the blood of West Nile virus-infected donors / M. Throsby and J. de Kruif
Anti-beta(2)GP-I and anti-prothrombin antibodies generated by phage display / J. Cabiedes and A.R. Cabral
The generation and selection of single-domain, v region libraries from nurse sharks / M.F. Flajnik and H. Dooley
The isolation of scFvs against small target molecules / I. Shaw and M. Kane
Display and selection of scFv antibodies on HEK-293T cells / M. Ho and I. Pastan
Isolation of scFvs that inhibit the NS3 protease of hepatitis C virus by a combination of phage display and a bacterial genetic screen / L. Nahary, A. Trahtenherts and I. Benhar
Guided selection methods through chain shuffling / B. Guo-Qiang and H. Xian-Li
Sequential antigen panning for selection of broadly cross-reactive HIV-1-neutralizing human monoclonal antibodies / M.Y. Zhang and D.S. Dimitrov
Selection of antibodies able to rapidly enter mammalian cells / M.A. Poul
Identification of the specificity of isolated phage display single-chain antibodies using yeast two-hybrid screens / N. Rasmussen and H.J. Ditzel
Generation of bispecific and tandem diabodies / S.M. Kipriyanov
Intrabody expression in eukaryotic cells / L. Guglielmi and P. Martineau
High-level periplasmic expression and purification of scFvs / S.M. Kipriyanov
Expression of single-chain Fv fragments in E. coli cytoplasm / L. Guglielmi and P. Martineau
High-level expression of a phage display-derived scFv in Pichia pastoris / L.M. Damasceno ... [et al.].

Edition Notes

Includes bibliographical references and index.

Mode of access: Internet.

Methods in molecular biology -- 562, Springer protocols


Dewey Decimal Class
Library of Congress

The Physical Object

x, 240 p. :
Number of pages

ID Numbers

Open Library
Internet Archive
1603273018, 1603273026
9781603273015, 9781603273022
LC Control Number


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