An edition of In situ hybridization protocols
(2005)
In situ hybridization protocols
3rd ed.
by Ian A. Darby and Tim D. Hewitson
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This revised and updated edition emphasizes tissue and cell in situ hybridization methods. Among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. There are also cutting-edge techniques for tissue microarrays, expanded embryology-developmental gene detection, and expanded cell culture. Derivative techniques presented include identification of transplanted cells, histones, nick-end labeling for apoptosis, the use of peptide nucleic acid probes, and in situ hybridization of plant specimens.
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Table of Contents
Treatment of tissue sections for in situ hybridization / Gregory H. Tesch, Hui Y. Lan, and David J. Nikolic-Paterson
Probe design / Peter Roche
cRNA probes: comparison of isotopic and nonisotopic detection methods / Teresa Bisucci, Tim D. Hewitson, and Ian A. Darby
Tyramide signal amplification for DNA and mRNA in situ hybridization / Ernst J.M. Speel, Anton H.N. Hopman, and Paul Komminoth
Expression analysis of murine genes using in situ hybridization with radioactive and non-radioactively labeled probes / Anne Chotteau-Lelièvre, Pascal Dollé, and Françoise Gofflot
Non-radioactive in situ hybridization on frozen sections and whole mounts / Julia Asp, Alexandra Abramsson, and Christer Betsholtz
In situ hybridization of whole mount embryos / Murray Hargrave, Josephine Bowles, and Peter Koopman
In situ detection of Epstein-Barr virus and phenotype determination of EBV-infected cells / Gerald Niedobitek and Hermann Herbst
PNA- situ hybridization method for detection of HIV-1 DNA in virus-infected cells and subsequent detection of cellular and viral proteins / Tomoko Hagiwara, Junko Hattori, and Tsuguhiro Kaneda
Electron microscopy and in situ hybridization: expression of P2Y2 receptor mRNA in the cerebellum / Andrzej Loesch and Rainer Glass
Free-floating sections of brain in situ / Neil Owens
An in situ hybridization technique to detect low-abundance slug mRNA in adherent cultured cells / Changsun Choi ... [et al.]
Identification of transplanted human cells in animal tissues / Daniel Benten, Kang Cheng, and Sanjeev Gupta
In situ hybridisation to plant tissues and chromosomes / Andreas Houben, Sharon J. Orford, and Jeremy N. Timmis
Histochemical localization of cell proliferation using in situ hybridization for histone mRNA / Tim D. Hewitson, Kristen J. Kelynack, and Ian A. Darby
Histochemical localization of apoptosis with in situ labeling of fragmented DNA / Tim D. Hewitson, Teresa Bisucci, and Ian A. Darby
The use of combined immunohistochemical labeling and in situ hybridization to co-localize mRNA and protein in tissue sections / Malcolm D. Smith, Michael Ahern, and Mark Coleman
Semi-quantitative in situ hybridization using radioactive probes to study gene expression in motoneuron populations / Paul D. Storer and Tracey DeLucia
Quantitative in situ hybridization of tissue microarrays / Adrian M. Jubb ... [et al.].
Edition Notes
Includes bibliographical references and index.
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