An edition of YAC protocols
(2006)
YAC protocols
2nd ed
by Alasdair MacKenzie
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Subjects
Genetic Techniques, Molecular cloning, Methods, Genetic vectors, Yeast fungi, Genetic engineering, Chromosomes, Artificial, Yeast, Genetic Vectors, Saccharomyces cerevisiae, Molecular cloning -- Methods, Yeast fungi -- Genetic engineering, Genetics, technique, Methodology, Fungi, genetics, YeastShowing 2 featured editions. View all 2 editions?
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Table of Contents
Markers, selection, and media in yeast artificial chromosomes cloning / David Markie
Construction of yeast artificial chromosomes libraries from pathogens and nonmodel organisms / Cecilia P. Sanchez and Michael Lanzer
Analysis and screening of yeast artificial chromosomes libraries by filter hybridization / Cecilia P. Sanchez and Michael Lanzer
Analysis of yeast artificial chromosome DNA by restriction digestion, southern blotting nucleic acid hybridization and polymerase chain reaction / Sylvia Vasiliou and John Peter Quinn
Postgenomic bioinformatic analysis of yeast artificial chromosomes sequence / Kerry Miller, Scott Davidson, and Alasdair MacKenzie
Transformation of yeast using bio-active beads with surface-immobilized yeast artificial chromosomes / Shigeki Kawakami ... [et al.]
Modification and amplification of yeast artificial chromosomes / Sanbing Shen, Anthony Harmar, and Nick Hastie
Modifying yeast artificial chromosomes to generate Cre/LoxP and FLP/FRT site-specific deletions and inversions / Gabriela G. Loots
Selective isolation of large chromosomal regions by transformation-associated recombination cloning for structural and functional analysis of mammalian genomes / Natalay Kouprina, Vladimir N. Noskov, and Vladimir Larionov
A polymerase chain reaction-mediated yeast artificial chromosomes-splitting technology for generating targeted yeast artificial chromosomes subclones / Yeon-Hee Kim ... [et al.]
Genomic reconstruction by serial mitotic recombination of yeast artificial chromosomes / David Markie, Emma Jones, and Jiannis Ragoussis
Targeting vector construction by yeast artificial chromosome modification / Peter J. Murray
Production of yeast artificial chromosomes transgenic mice by pronuclear injection of one-cell embryos / Alasdair MacKenzie
Generation of yeast artificial chromosomes transgenic mice by intracytoplasmic sperm injection / Pedro N. Moreira ... [et al.]
Establishment of cell lines that exhibit correct ontogenic stage-specific gene expression pprofiles from tissues of yeast artificial chromosome transgenic mice using chemically induced growth signals / C. Anthony Blau and Kenneth R. Peterson
Application of yeast artificial chromosomes in fluorescence in situ hybridization / Thomas Liehr
Contribution of yeast artificial chromosome-based physical maps to the final assembly of the trypanosoma cruzi genome / Márcia R.M. Santos ... [et al.]
Edition Notes
Includes bibliographical references and index
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