Shwachman-Diamond Syndrome (SDS) is an autosomal recessive disorder with clinical features that include pancreatic exocrine insufficiency, hematological dysfunction and skeletal abnormalities. The SBDS gene mutated in this disorder is highly conserved in archaea and all eukaryotes. Although its function remains unknown, there is indirect evidence suggesting involvement in RNA metabolism. Strategies were attempted to identify protein and RNA interactors of SBDS to gain insight into its function. Both specific testing and broad yeast two-hybrid screens of disease relevant tissues failed to identify direct protein binding partners. An affinity binding assay also did not yield direct interactors. A single RNA binding experiment was attempted, but it also failed to identify specifically bound molecules. Investigations to firmly establish localization and alternate strategies that incorporate covalent cross-linking procedures will be needed to elucidate the function of SBDS.