Microchannel fabrication methods and the particle-liquid flow characteristics in a microfluidic system were investigated experimentally. Combination of heat press and heat bonding was tested on polycarbonate and acrylic. In heat press, the best pressing temperatures for polycarbonate and acrylic were found to be 135°C and 65°C, respectively; and the optimum pressing times were both 120 minutes. In heat bonding, the best bonding temperatures for polycarbonate and acrylic were 135°C and 60°C at bonding times of 150 and 90 minutes, respectively.In the particle-liquid flow experiments, the effect of stirring in the inlet reservoir on the particle flow in a 75mum-diameter microchannel was examined. The number of particles that entered and flowed through the microchannel was found to increase with the liquid flow rate but decrease with stirring rate. Stirring in the reservoir was found to be detrimental to particle-liquid flow since it generated particle clusters within the reservoir.