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Book Details
Table of Contents
The transcriptional landscape
Working with RNA
Synthesis of RNA by in vitro transcription
Efficient poly(A)+ RNA selection using LNA oligo(T) capture
Genome browsers
Web-based tools for studying RNA structure and function
Northern blotting analysis
Rapid amplification of cDNA ends (RACE)
Fractionation of mRNA based on the length of the poly(A) tail
Analysis of mutations that influence pre-mRNA splicing
S1 nuclease analysis of alternatively spliced mRNA
Promises and challenges in developing RNAi as a research tool and therapy
Inhibition of gene function in mammalian cells using short-hairpin RNA (shRNA)
Validation of RNAi by real time PCR
Profiling RNA polymerase II using the fast chromatin immunoprecipitation method
The post-transcriptional operon
RIP-chip analysis : RNA-binding protein immunoprecipitation-microarray (chip) profiling
Isolation of RNP granules
Electrophoretic mobility shift assay for characterizing RNA-protein interaction
Polysome analysis and RNA purification from sucrose gradients
Prediction of targets for microRNAs
Outsourcing of experimental work.
Edition Notes
Includes bibliographical references and index.
The Physical Object
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