An edition of RNA (2011)

RNA

methods and protocols

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RNA
Henrik Nielsen, Henrik Nielsen
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Last edited by MARC Bot
December 24, 2022 | History
An edition of RNA (2011)

RNA

methods and protocols

  • 1 Want to read

This edition doesn't have a description yet. Can you add one?

Publish Date
Publisher
Humana Press
Language
English
Pages
329

Buy this book

Edition Availability
Cover of: RNA
RNA: Methods and Protocols
2017, Humana Press
in English
Cover of: RNA
RNA: methods and protocols
2011, Humana Press
in English

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Book Details


Table of Contents

The transcriptional landscape
Working with RNA
Synthesis of RNA by in vitro transcription
Efficient poly(A)+ RNA selection using LNA oligo(T) capture
Genome browsers
Web-based tools for studying RNA structure and function
Northern blotting analysis
Rapid amplification of cDNA ends (RACE)
Fractionation of mRNA based on the length of the poly(A) tail
Analysis of mutations that influence pre-mRNA splicing
S1 nuclease analysis of alternatively spliced mRNA
Promises and challenges in developing RNAi as a research tool and therapy
Inhibition of gene function in mammalian cells using short-hairpin RNA (shRNA)
Validation of RNAi by real time PCR
Profiling RNA polymerase II using the fast chromatin immunoprecipitation method
The post-transcriptional operon
RIP-chip analysis : RNA-binding protein immunoprecipitation-microarray (chip) profiling
Isolation of RNP granules
Electrophoretic mobility shift assay for characterizing RNA-protein interaction
Polysome analysis and RNA purification from sucrose gradients
Prediction of targets for microRNAs
Outsourcing of experimental work.

Edition Notes

Includes bibliographical references and index.

Published in
New York, N.Y
Series
Springer Protocols, Methods in molecular biology -- 703, Springer protocols (Series), Methods in molecular biology (Clifton, N.J.) -- v. 703.
Copyright Date
2011

The Physical Object

Pagination
xv, 329 pages
Number of pages
329

Edition Identifiers

Open Library
OL44892066M
OCLC/WorldCat
695401774

Work Identifiers

Work ID
OL26499722W

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