An edition of Cell biology assays
(2009)
Cell biology assays
essential methods
by Geri Kreitzer and J. E. Celis
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Table of Contents
Preface p. vii
List of Contributors p. ix
Membrane Protein Translocation Assays: Exocytosis, Endocytosis, Er-Golgi, Translocation Through the Er Permeabilized Epithelial Cells to Study Exocytic Membrane Transport p. 3
Studying Exit and Surface Delivery of Post-Golgi Transport Intermediates Using In Vitro and Live-Cell Microscopy-Based Approaches p. 13
Use of Permeabilized Mast Cells to Analyze Regulated Exocytosis p. 29
Cell Surface Biotinylation and Other Techniques for Determination of Surface Polarity of Epithelial Monolayers p. 39
Assays Measuring Membrane Transport in the Endocytic Pathway p. 53
Microsome-Based Assay for Analysis of Endoplasmic Reticulum to Golgi Transport in Mammalian Cells p. 63
Cotranslational Translocation of Proteins into Microsomes Derived from the Rough Endoplasmic Reticulum of Mammalian Cells p. 71
Loading Protein into Cells to Assess PM Function Syringe Loading: A Method for Assessing Plasma Membrane Function as a Reflection of Mechanically Induced Cell Loading p. 83
Protein Translocation Between Cytoplasm and Compartments Other than Plasma Membrane Protein Translocation into Mitochondria p. 97
Polarographic Assays of Mitochondrial Functions p. 107
Analysis of Nuclear Protein Import and Export in Digitonin-Permeabilized Cells p. 115
Heterokaryons: An Assay for Nucleocyto-plasmic Shuttling p. 127
Measuring Intracellular Signaling Events Using Fluorescence Microscopy Ca2+ as a Second Messenger: New Reporters for Calcium (Cameleons and Camgaroos) p. 139
Ratiometric Pericam p. 153
In Situ Cell Electroporation to Study of Signaling Pathways Dissecting Pathways; In Situ Electroporation for the Study of Signal Transduction and Gap Junctional Communication p. 165
In Situ Electroporation of Radioactive Nucleotides: Assessment of Ras Activity or 32P Labeling of Cellular Proteins p. 183
Tracking Individual Chromosomes with Integrated Arrays of lacop Sites and GFP-laci Repress.
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- Created July 30, 2014
- 4 revisions
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| October 9, 2020 | Edited by ImportBot | import existing book |
| August 2, 2020 | Edited by ImportBot | import existing book |
| May 23, 2020 | Edited by CoverBot | Added new cover |
| July 30, 2014 | Created by ImportBot | Imported from Internet Archive item record |