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"DNA Sequencing: Optimizing the Process and Analysis was written to help scientists sequence difficult templates. It is also a practical guide to faster and more efficient routine DNA sequencing. This in-depth volume covers important topics in the field, including biochemical and technological advances stemming from the Human Genome Project, proven and newly emerging methods of preparing DNA templates, the effects of widely used laboratory reagents on DNA sequencing, Laboratory Information Management Systems (LIMS), setting up of a GLP/GMP DNA sequencing lab, and the future of DNA sequencing."--BOOK JACKET.
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Edition | Availability |
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DNA sequencing: optimizing the process and analysis
2005, Jones and Bartlett Publishers
in English
0763747823 9780763747824
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Table of Contents
Controlled Heat-Denaturation of DNA Plasmids / Jan Kieleczawa
Effects of Various Reagents on DNA Sequencing Process / Jan Kieleczawa
Sequencing of Difficult DNA Templates / Jan Kieleczawa
New DNA Sequencing Enzymes / Sandra L. Spurgeon and John W. Brandis
Beyond pUC: Vectors for Cloning Unstable DNA / Ronald Godiska, Melodee Patterson, Tom Schoenfeld, and David A. Mead
Recombination-Based Cloning / Vincent Ling
Plasmid Preparation Methods for DNA Sequencing / Parke K. Flick
Optimization of Culture Growth in 96-Deep-Well Plates / Jan Kieleczawa
Automated DNA Scanners Used in Sequencing Laboratories / Jan Kieleczawa
Geospiza's Finch-Server: A Complete Data Management System for DNA Sequencing / Sandra Porter, Joseph Slagel, and Todd Smith
DNA Sequencing Database: A Flexible LIMS for DNA Sequencing Analysis / Donald M. Koffman and Hemchand Sookdeo
Good Laboratory Practices, Good Manufacturing Procedures, and Quality Assurance in the DNA Sequencing Laboratory / Michele Godlevski and Thalia Taylor
Future of DNA Sequencing: Towards an Affordable Genome / Kevin McKeman
Edition Notes
Includes bibliographical references and index
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