Functional importance of Munc18-1 and its interaction with syntaxin1 in PC12 cells.

Functional importance of Munc18-1 and its int ...
Lakshmanan Arunachalam, Lakshm ...
Locate

My Reading Lists:

Create a new list



Buy this book

Last edited by WorkBot
January 24, 2010 | History

Functional importance of Munc18-1 and its interaction with syntaxin1 in PC12 cells.

Munc18-1 is a 67kDa mammalian orthologue to unc18 in C.elegans , which was first identified as a syntaxin binding protein. Deletion of Munc18-1 results in severely hampered exocytosis in neurons and chromaffin cells. Utilising RNA interference, we have established PC12 cells in which Munc18-1 is stably knocked down by more than 90%. Our secretion assays revealed a 40-70% decrease in Ca-dependent vesicle exocytosis in the Munc18-1 Knockdown (KD) cells. In addition there was also a 40-50% decrease in the proportion of docked vesicles within Munc18-1 KD PC12 cells. Analysis of the localization of syntaxin1 in Munc18-1 KD cells reveals a significant intracellular mislocalization compared to controls. Such syntaxin1 mislocalization was also nearly completely rescued upon reintroduction of Munc18-1 into the Munc18-1 KD PC12 cells. This clearly supports our hypothesis that Munc18-1 acts as a molecular chaperone to syntaxin1 to facilitate the transport of syntaxin1 to the cell membrane in PC12 cells.

Publish Date
Language
English
Pages
101

Buy this book

Book Details


Edition Notes

Source: Masters Abstracts International, Volume: 44-02, page: 0768.

Thesis (M.Sc.)--University of Toronto, 2005.

Electronic version licensed for access by U. of T. users.

GERSTEIN MICROTEXT copy on microfiche (2 microfiches).

The Physical Object

Pagination
101 leaves.
Number of pages
101

Edition Identifiers

Open Library
OL19217755M
ISBN 10
0494074876

Work Identifiers

Work ID
OL12683943W

Community Reviews (0)

No community reviews have been submitted for this work.

Lists

History

Download catalog record: RDF / JSON
January 24, 2010 Edited by WorkBot add more information to works
December 11, 2009 Created by WorkBot add works page