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January 24, 2010 | History

Functional importance of Munc18-1 and its interaction with syntaxin1 in PC12 cells 1 edition

Functional importance of Munc18-1 and its interaction with syntaxin1 i ...
Lakshmanan Arunachalam

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Functional importance of Munc18-1 and its interaction with syntaxin1 in PC12 cells.

Published 2005 .
Written in English.

About the Book

Munc18-1 is a 67kDa mammalian orthologue to unc18 in C.elegans , which was first identified as a syntaxin binding protein. Deletion of Munc18-1 results in severely hampered exocytosis in neurons and chromaffin cells. Utilising RNA interference, we have established PC12 cells in which Munc18-1 is stably knocked down by more than 90%. Our secretion assays revealed a 40-70% decrease in Ca-dependent vesicle exocytosis in the Munc18-1 Knockdown (KD) cells. In addition there was also a 40-50% decrease in the proportion of docked vesicles within Munc18-1 KD PC12 cells. Analysis of the localization of syntaxin1 in Munc18-1 KD cells reveals a significant intracellular mislocalization compared to controls. Such syntaxin1 mislocalization was also nearly completely rescued upon reintroduction of Munc18-1 into the Munc18-1 KD PC12 cells. This clearly supports our hypothesis that Munc18-1 acts as a molecular chaperone to syntaxin1 to facilitate the transport of syntaxin1 to the cell membrane in PC12 cells.

Edition Notes

Source: Masters Abstracts International, Volume: 44-02, page: 0768.

Thesis (M.Sc.)--University of Toronto, 2005.

Electronic version licensed for access by U. of T. users.

GERSTEIN MICROTEXT copy on microfiche (2 microfiches).

The Physical Object

Pagination
101 leaves.
Number of pages
101

ID Numbers

Open Library
OL19217755M
ISBN 10
0494074876

History Created December 11, 2009 · 2 revisions Download catalog record: RDF / JSON

January 24, 2010 Edited by WorkBot add more information to works
December 11, 2009 Created by WorkBot add works page