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January 24, 2010 | History

Functional importance of Munc18-1 and its interaction with syntaxin1 in PC12 cells 1 edition

Functional importance of Munc18-1 and its interaction with syntaxin1 i ...
Lakshmanan Arunachalam

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Functional importance of Munc18-1 and its interaction with syntaxin1 in PC12 cells.

Published 2005 .
Written in English.

About the Book

Munc18-1 is a 67kDa mammalian orthologue to unc18 in C.elegans , which was first identified as a syntaxin binding protein. Deletion of Munc18-1 results in severely hampered exocytosis in neurons and chromaffin cells. Utilising RNA interference, we have established PC12 cells in which Munc18-1 is stably knocked down by more than 90%. Our secretion assays revealed a 40-70% decrease in Ca-dependent vesicle exocytosis in the Munc18-1 Knockdown (KD) cells. In addition there was also a 40-50% decrease in the proportion of docked vesicles within Munc18-1 KD PC12 cells. Analysis of the localization of syntaxin1 in Munc18-1 KD cells reveals a significant intracellular mislocalization compared to controls. Such syntaxin1 mislocalization was also nearly completely rescued upon reintroduction of Munc18-1 into the Munc18-1 KD PC12 cells. This clearly supports our hypothesis that Munc18-1 acts as a molecular chaperone to syntaxin1 to facilitate the transport of syntaxin1 to the cell membrane in PC12 cells.

Edition Notes

Source: Masters Abstracts International, Volume: 44-02, page: 0768.

Thesis (M.Sc.)--University of Toronto, 2005.

Electronic version licensed for access by U. of T. users.

GERSTEIN MICROTEXT copy on microfiche (2 microfiches).

The Physical Object

101 leaves.
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History Created December 11, 2009 · 2 revisions Download catalog record: RDF / JSON

January 24, 2010 Edited by WorkBot add more information to works
December 11, 2009 Created by WorkBot add works page