Repair of slipped (CAG)·(CTG) structures by undifferentiated and differentiated human neuron-like cells.

Repair of slipped (CAG)·(CTG) structures by u ...
Michelle Blondin, Michelle Blo ...
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January 24, 2010 | History

Repair of slipped (CAG)·(CTG) structures by undifferentiated and differentiated human neuron-like cells.

Instability of trinucleotide repeat sequences such as (CAG)•(CTG) have been linked to more than 14 human disorders, including Huntington disease and myotonic dystrophy. Repeat instability is thought to arise through the escaped/failed or aberrant repair of slipped DNA repeat structures. Since many trinucleotide repeat disorders display a neurological phenotype, we sought to investigate whether slipped CAG and CTG repeat structures could be repaired by cell extracts of the human neuroblastoma cell line, SH-SY5Y. Extracts of undifferentiated and differentiated SH-SY5Y cells were able to repair slipped CAG and CTG repeat structures in a nick-directed fashion, with no quantitative or qualitative differences in repair efficiencies between undifferentiated and differentiated SH-SY5Y extracts. However, repair efficiency was influenced by nick polarity as structures with 5' nicks displayed higher repair efficiencies than those with 3' nicks. Such repair influences may help to explain the different mutation patterns seen at various genetic loci and tissues.

Publish Date
Language
English
Pages
85

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Book Details


Edition Notes

Source: Masters Abstracts International, Volume: 44-02, page: 0760.

Thesis (M.Sc.)--University of Toronto, 2005.

Electronic version licensed for access by U. of T. users.

GERSTEIN MICROTEXT copy on microfiche (1 microfiche).

The Physical Object

Pagination
85 leaves.
Number of pages
85

Edition Identifiers

Open Library
OL19217301M
ISBN 10
0494073926

Work Identifiers

Work ID
OL12683700W

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January 24, 2010 Edited by WorkBot add more information to works
December 11, 2009 Created by WorkBot add works page