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January 24, 2010 | History

Functional studies of Lats2 1 edition

Functional studies of Lats2
Laura Tamblyn

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Functional studies of Lats2.

Published 2005 .
Written in English.

About the Book

Lats2 is a putative tumour suppressor that may have roles in regulating cell cycle progression, centrosome duplication, and apoptosis. This thesis describes the generation of tools for characterizing Lats2 function: a Tandem Affinity Purification (TAP) system to identify novel Lats2-interacting proteins and a conditional knock-out mouse to study the in vivo role of Lats2. I cloned a TAP-Lats2 expression plasmid, generated stable TAP-Lats2 NIH3T3 cell lines and used the TAP method to purify TAP-Lats2 protein. I partially optimized the purification method and assayed for proper function of the TAP-Lats2 protein. For concurrent in vivo studies, I generated mouse embryo stem cells and chimeric mice carrying a Lats2 allele with a floxed Exon 2 . In future research, the CRE/LoxP system can be used in vivo to interupt Lats2 expression from the floxed allele in an inducible and tissue-specific manner. These tools will help define Lats2's role in molecular pathways and tumorigenesis.

Edition Notes

Source: Masters Abstracts International, Volume: 44-02, page: 0767.

Thesis (M.Sc.)--University of Toronto, 2005.

Electronic version licensed for access by U. of T. users.

GERSTEIN MICROTEXT copy on microfiche (1 microfiche).

The Physical Object

62 leaves.
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History Created December 11, 2009 · 2 revisions Download catalog record: RDF / JSON

January 24, 2010 Edited by WorkBot add more information to works
December 11, 2009 Created by WorkBot add works page