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January 24, 2010 | History

Functional studies of Lats2 1 edition

Functional studies of Lats2
Laura Tamblyn

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Functional studies of Lats2.

Published 2005 .
Written in English.

About the Book

Lats2 is a putative tumour suppressor that may have roles in regulating cell cycle progression, centrosome duplication, and apoptosis. This thesis describes the generation of tools for characterizing Lats2 function: a Tandem Affinity Purification (TAP) system to identify novel Lats2-interacting proteins and a conditional knock-out mouse to study the in vivo role of Lats2. I cloned a TAP-Lats2 expression plasmid, generated stable TAP-Lats2 NIH3T3 cell lines and used the TAP method to purify TAP-Lats2 protein. I partially optimized the purification method and assayed for proper function of the TAP-Lats2 protein. For concurrent in vivo studies, I generated mouse embryo stem cells and chimeric mice carrying a Lats2 allele with a floxed Exon 2 . In future research, the CRE/LoxP system can be used in vivo to interupt Lats2 expression from the floxed allele in an inducible and tissue-specific manner. These tools will help define Lats2's role in molecular pathways and tumorigenesis.

Edition Notes

Source: Masters Abstracts International, Volume: 44-02, page: 0767.

Thesis (M.Sc.)--University of Toronto, 2005.

Electronic version licensed for access by U. of T. users.

GERSTEIN MICROTEXT copy on microfiche (1 microfiche).

The Physical Object

Pagination
62 leaves.
Number of pages
62

ID Numbers

Open Library
OL19217297M
ISBN 10
049407390X

History Created December 11, 2009 · 2 revisions Download catalog record: RDF / JSON

January 24, 2010 Edited by WorkBot add more information to works
December 11, 2009 Created by WorkBot add works page