Lats2 is a putative tumour suppressor that may have roles in regulating cell cycle progression, centrosome duplication, and apoptosis. This thesis describes the generation of tools for characterizing Lats2 function: a Tandem Affinity Purification (TAP) system to identify novel Lats2-interacting proteins and a conditional knock-out mouse to study the in vivo role of Lats2. I cloned a TAP-Lats2 expression plasmid, generated stable TAP-Lats2 NIH3T3 cell lines and used the TAP method to purify TAP-Lats2 protein. I partially optimized the purification method and assayed for proper function of the TAP-Lats2 protein. For concurrent in vivo studies, I generated mouse embryo stem cells and chimeric mice carrying a Lats2 allele with a floxed Exon 2 . In future research, the CRE/LoxP system can be used in vivo to interupt Lats2 expression from the floxed allele in an inducible and tissue-specific manner. These tools will help define Lats2's role in molecular pathways and tumorigenesis.