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January 24, 2010 | History

Modulation of culture conditions to improve cartilaginous tissue formed in vitro 1 edition

Modulation of culture conditions to improve cartilaginous tissue forme ...
David Cajetan Couto

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Modulation of culture conditions to improve cartilaginous tissue formed in vitro.

Published 2005 .
Written in English.

About the Book

Cartilage has limited ability for repair and one possible treatment would be tissue engineering. However cartilage tissue formed in vitro is biochemically and mechanically inferior when compared to native tissue. I hypothesized that modulation of culture conditions will improve cartilage tissue formation in vitro. Supplementing Ham's F12 culture media with 14mM NaHCO3 to maintain pH in the neutral zone resulted in more cartilage which showed cellular organization. Application of compressive and shear forces also resulted in more cartilage formation with improved weight bearing ability by increasing expression of collagen and proteoglycan gene expression. Further study is required to determine the optimal culture conditions.

Edition Notes

Source: Masters Abstracts International, Volume: 44-02, page: 0740.

Thesis (M.Sc.)--University of Toronto, 2005.

Electronic version licensed for access by U. of T. users.

GERSTEIN MICROTEXT copy on microfiche (2 microfiches).

The Physical Object

117 leaves.
Number of pages

ID Numbers

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January 24, 2010 Edited by WorkBot add more information to works
December 11, 2009 Created by WorkBot add works page