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January 24, 2010 | History

Local microenvironmental control of developing embryoid bodies into hematopoietic progenitor cells in a serum-free defined culture condition 1 edition

Local microenvironmental control of developing embryoid bodies into he ...
Jennifer G. Morin

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Local microenvironmental control of developing embryoid bodies into hematopoietic progenitor cells in a serum-free defined culture condition.

Published 2005 .
Written in English.

About the Book

Use of embryonic stem (ES) cells in regenerative medicine requires the development of robust clinically relevant bioprocesses for the production of ES-derived cells. We improved upon current mouse ES cell culture methods by developing a serum-free defined culture system amenable to controlled cytokine delivery. We enhanced blood development by specifying the quantity and timing of bone morphogenetic protein (BMP)-4, vascular endothelial growth factor and thrombopoietin exposure. We also developed a cytokine delivery system for growing embryoid bodies (EBs) based on the binding of BMP-4 to functionalized agarose microcapsules. We demonstrated that immobilized BMP-4 can be delivered to the developing EB, inducing at least 60% of the cells to express mesoderm-associated markers. These results suggest that this delivery system could be used for the development of scalable bioengineered niche for EB-based blood generation. This process represents a cost effective and robust platform for generating ES cell-derived functional cell types.

Edition Notes

Source: Masters Abstracts International, Volume: 44-02, page: 0960.

Thesis (M.A.Sc.)--University of Toronto, 2005.

Electronic version licensed for access by U. of T. users.

ROBARTS MICROTEXT copy on microfiche.

The Physical Object

Pagination
94 leaves.
Number of pages
94

ID Numbers

Open Library
OL19216181M
ISBN 10
0494071109

History Created December 11, 2009 · 2 revisions Download catalog record: RDF / JSON

January 24, 2010 Edited by WorkBot add more information to works
December 11, 2009 Created by WorkBot add works page