A genetic reporter/selection system for optimization of lineage specific embryonic stem cell differentiation.

A genetic reporter/selection system for optim ...
Cyrus Z. Handy, Cyrus Z. Handy
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January 24, 2010 | History

A genetic reporter/selection system for optimization of lineage specific embryonic stem cell differentiation.

Enhancing the ability of conventional tissue culture techniques to produce specific differentiated cell types, requires an approach that is based upon manipulation of extrinsic and intrinsic signalling pathways combined with selection techniques. As a first step we have developed a series of genetic reporter / selection constructs, which will permit quantitative analysis of lineage-specific differentiation, critical for optimization of tissue engineering protocols. The reporters impart differential fluorescence and antibiotic resistance to ESC-derived cells; such that cell lines can be created that allow the marking and selection of, and discrimination between, cells that are expressing the gene of interest and the progeny of those cells. Our first target is the T-box transcription factor Brachyury (T), which is transiently expressed by nascent early mesoderm progenitors. Our T reporter cell lines will allow us to visualize and manipulate mesoderm development, enable the isolation and characterization of primitive mesoderm progenitors or mesoderm derived cell lines.

Publish Date
Language
English
Pages
128

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Book Details


Edition Notes

Source: Masters Abstracts International, Volume: 44-02, page: 0957.

Thesis (M.A.Sc.)--University of Toronto, 2005.

Electronic version licensed for access by U. of T. users.

GERSTEIN MICROTEXT copy on microfiche (2 microfiches).

The Physical Object

Pagination
128 leaves.
Number of pages
128

Edition Identifiers

Open Library
OL19216157M
ISBN 10
0494071036

Work Identifiers

Work ID
OL12683182W

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January 24, 2010 Edited by WorkBot add more information to works
December 11, 2009 Created by WorkBot add works page