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Last edited by WorkBot
January 24, 2010 | History

Subcellular localization of human Nedd4-2 splice isoforms 1 edition

Subcellular localization of human Nedd4-2 splice isoforms
Kathleen Nethery-Brokx

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Subcellular localization of human Nedd4-2 splice isoforms.

Published 2006 .
Written in English.

About the Book

Neural precursor cell-expressed developmentally downregulated 4 (Nedd4) is an E3 ubiquitin-protein ligase that has an N-terminal C2 domain, three or four WW domains and a C-terminal HECT domain. The C2 domain is a small (∼130 amino acid) calcium binding, lipid-binding and protein-protein interaction domain. In polarized MDCK cells V5 epitope-tagged human Nedd4-2(+C2) and hNedd4-2(+C2) were used for both confocal and EM experiments. hNedd4-2(+C2) localized to the apical and lateral membranes of MDCK cells both in the presence and absence of increased cystolic calcium levels, and the hNedd4-2(DeltaC2) isoform demonstrated cystolic localization. Binding of GST-tagged C2 domains from rat Nedd4-1, hNedd4-1 and hNedd4-2 to nitrocellulose-bound phospholipids showed binding of all C2 domains to phosphatidylinositols (PtdIns) that increased with addition of calcium. This study has provided evidence that the C2 domain of hNedd4-2 serves to target the protein to the apical membrane of polarized epithelium where it can interact with its substrates.

Edition Notes

Source: Masters Abstracts International, Volume: 44-06, page: 2803.

Thesis (M.Sc.)--University of Toronto, 2006.

Electronic version licensed for access by U. of T. users.

ROBARTS MICROTEXT copy on microfiche.

The Physical Object

Pagination
116 leaves.
Number of pages
116

ID Numbers

Open Library
OL19214614M
ISBN 13
9780494160657

History Created December 11, 2009 · 2 revisions Download catalog record: RDF / JSON

January 24, 2010 Edited by WorkBot add more information to works
December 11, 2009 Created by WorkBot add works page