Basic methods in protein purification and analysis

a laboratory manual

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Last edited by ImportBot
June 18, 2024 | History

Basic methods in protein purification and analysis

a laboratory manual

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  • 0 Currently reading
  • 0 Have read

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Publish Date
Language
English

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Edition Availability
Cover of: Basic methods in protein purification and analysis
Basic methods in protein purification and analysis: a laboratory manual
2008, Cold Spring Harbor Laboratory Press
in English

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Book Details


Table of Contents

Introduction to protein purification strategies
Preparation of cellular and subcellular extracts
One-dimensional polyacrylamide gel electrophoresis
Preparative two-dimensional gel electrophoresis with immobilized PH gradients
Ion-exchange chromatography
Size-exclusion chromatography
Reversed-phase high-performance liquid chromatography
Affinity and immunoaffinity chromatography
Metal chelate affinity chromatography
Multidimensional chromatography of intact proteins
Identification of protein-protein interactions by conventional column chromatography
Identification of associated proteins by coimmunoprecipitation
Chromatin immunoprecipitation of protein complexes
Identification of protein-protein interactions with glutathione-s-transferase fusion proteins
Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis
In silico tools to launch protein interaction analysis : creating focused networks around proteins of interest.

Edition Notes

Includes bibliographical references and index.

Published in
Cold Spring Harbor, N.Y
Genre
Laboratory manuals, Laboratory Manuals

Classifications

Dewey Decimal Class
572/.6078
Library of Congress
QP551 .B265 2008, QP551 .B265 2009, QP551.B265 2008

The Physical Object

Pagination
p. ;

ID Numbers

Open Library
OL23159780M
ISBN 13
9780879698683, 9780879698676
LCCN
2008031690
Goodreads
5039657

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History

Download catalog record: RDF / JSON / OPDS | Wikipedia citation
June 18, 2024 Edited by ImportBot import existing book
December 7, 2022 Edited by ImportBot import existing book
October 30, 2022 Edited by ImportBot import existing book
September 16, 2021 Edited by ImportBot import existing book
May 11, 2009 Created by ImportBot Imported from Library of Congress MARC record