MARC Record from University of Toronto

LEADER: 02076nam 2200265 4500
001 AAIMR16067
005 20070119093939.5
008 070119s2006 onc|||||||||||||| ||eng d
020 $a9780494160671
039 $feh
100 1 $aHolmes, Christina.
245 10 $aLongitudinal characterization of the mesenchymal progenitor cell phenotype of Sca-1/Ly6A null mice.
260 $c2006.
300 $a174 leaves.
500 $aSource: Masters Abstracts International, Volume: 44-06, page: 2875.
502 $aThesis (M.A.Sc.)--University of Toronto, 2006.
506 $aElectronic version licensed for access by U. of T. users.
520 $aStem cell antigen-1 (Sca-1/Ly6A) is a GPI-AP expressed by a variety of stem cell populations. Previous studies involving Sca-1 knockout (KO) mice revealed an age-related osteoporotic phenotype, characterized by decreased bone mass and bone brittleness, due to mesenchymal progenitor defects. In this study, the in vitro mesenchymal progenitor cell phenotype of Sca-1-/- mice and wild type BALB/ c controls was characterized at 3, 5, 7, 9 and 12 months of age. A significant defect in osteo-, adipo- and total mesenchymal progenitors in Sca-1-/- mice was found to arise between 5 and 7 months of age, as measured by the CFU-A, CFU-ALP/O, and CFU-F assays, persisting until at least 12 months. These defects encompass progenitor self-renewal, expansion, and potentially osteogenic and adipogenic differentiation. These results provide the baseline parameters enabling the use of Sca-1-/- mice in future studies as model of age-related osteoporosis.
590 $aROBARTS MICROTEXT copy on microfiche.$5CaOTU
653 $aEngineering, Biomedical.
653 $aBiology, Cell.
653 $aBiology, Animal Physiology.
856 41 $uhttp://link.library.utoronto.ca/eir/EIRdetail.cfm?Resources__ID=441988&T=F$yConnect to resource
949 $atheses masters$wALPHANUM$c1$i6063689-1001$j2$lMICROTEXT$mMEDIA_COMM$rN$sY$tMICROFORM$u2/2/2007
949 $aOnline resource 441988$wASIS$c1$i6063689-2001$lONLINE$mE_RESOURCE$rY$sY$tE_RESOURCE$u2/2/2007