MARC Record from University of Toronto

LEADER: 02073nam 2200241 4500
001 AAIMR16065
005 20070119093939.5
008 070119s2006 onc|||||||||||||| ||eng d
020 $a9780494160657
039 $feh
100 1 $aNethery-Brokx, Kathleen.
245 10 $aSubcellular localization of human Nedd4-2 splice isoforms.
260 $c2006.
300 $a116 leaves.
500 $aSource: Masters Abstracts International, Volume: 44-06, page: 2803.
502 $aThesis (M.Sc.)--University of Toronto, 2006.
506 $aElectronic version licensed for access by U. of T. users.
520 $aNeural precursor cell-expressed developmentally downregulated 4 (Nedd4) is an E3 ubiquitin-protein ligase that has an N-terminal C2 domain, three or four WW domains and a C-terminal HECT domain. The C2 domain is a small (∼130 amino acid) calcium binding, lipid-binding and protein-protein interaction domain. In polarized MDCK cells V5 epitope-tagged human Nedd4-2(+C2) and hNedd4-2(+C2) were used for both confocal and EM experiments. hNedd4-2(+C2) localized to the apical and lateral membranes of MDCK cells both in the presence and absence of increased cystolic calcium levels, and the hNedd4-2(DeltaC2) isoform demonstrated cystolic localization. Binding of GST-tagged C2 domains from rat Nedd4-1, hNedd4-1 and hNedd4-2 to nitrocellulose-bound phospholipids showed binding of all C2 domains to phosphatidylinositols (PtdIns) that increased with addition of calcium. This study has provided evidence that the C2 domain of hNedd4-2 serves to target the protein to the apical membrane of polarized epithelium where it can interact with its substrates.
590 $aROBARTS MICROTEXT copy on microfiche.$5CaOTU
653 $aChemistry, Biochemistry.
856 41 $uhttp://link.library.utoronto.ca/eir/EIRdetail.cfm?Resources__ID=441986&T=F$yConnect to resource
949 $atheses masters$wALPHANUM$c1$i6063687-1001$j2$lMICROTEXT$mMEDIA_COMM$rN$sY$tMICROFORM$u2/2/2007
949 $aOnline resource 441986$wASIS$c1$i6063687-2001$lONLINE$mE_RESOURCE$rY$sY$tE_RESOURCE$u2/2/2007