MARC Record from Library of Congress

LEADER: 03597cam a22003617i 4500
001 2013956824
003 DLC
005 20140722081446.0
008 131204t20142014ne a b 000 0 eng d
010 $a 2013956824
020 $a9789400775442 (alk. paper)
020 $a940077544X (alk. paper)
020 $z9789400775459 (ebk.)
035 $a(OCoLC)ocn871645635
040 $aCDX$beng$cCDX$erda$dYDXCP$dBTCTA$dIXA$dSHH$dDLC
042 $alccopycat
050 00 $aQH212.F55$bM66 2014
100 1 $aMondal, Partha Pratim,$eauthor.
245 10 $aFundamentals of fluorescence microscopy :$bexploring life with light /$cPartha Pratim Mondal, Alberto Diaspro.
264 1 $aDordrecht ;$aNew York :$bSpringer,$c[2014]
264 4 $c©2014
300 $axv, 218 pages :$billustrations (some color) ;$c29 cm
336 $atext$btxt$2rdacontent
337 $aunmediated$bn$2rdamedia
338 $avolume$bnc$2rdacarrier
505 0 $aRay Optics, Wave Optics and Imaging System Design -- Basics of Electromagnetic Theory for Fluorescence Microscopy -- Electric Field Effects in Optical Microscopy Systems -- Quantum Description of Radiation Field and Optical Microscopy -- Molecular Physics of Fluorescent Markers -- Basics of Fluorescence and Photophysics -- General Fluorescence Imaging Techniques -- Multiphoton Fluorescence Microscopy -- Super-Resolution Fluorescence Microscopy -- Image Reconstruction for Fluorescence Microscopy -- Future Prospective of Fluorescence Microscopy.
520 $aThis book starts at an introductory level and leads reader to the most advanced developments in fluorescence imaging and super-resolution techniques that have enabled the emergence of new disciplines such as nanobioimaging, multiphoton microscopy, photodynamic therapy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going deep into the advanced imaging systems and their applications. Molecular orbital theory forms the basis for understanding fluorescent molecules and thereby facilitates complete explanation of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential fluorescence factors and phenomena such as, molecular cross-section, Stokes shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These phenomena form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part covers many imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction limited and unlimited imaging.--$cSource other than Library of Congress.
504 $aIncludes bibliographical references.
650 0 $aFluorescence microscopy.
700 1 $aDiaspro, Alberto,$d1959-$eauthor.
856 42 $3Contributor biographical information$uhttp://www.loc.gov/catdir/enhancements/fy1410/2013956824-b.html
856 42 $3Publisher description$uhttp://www.loc.gov/catdir/enhancements/fy1410/2013956824-d.html
856 41 $3Table of contents only$uhttp://www.loc.gov/catdir/enhancements/fy1410/2013956824-t.html