| Record ID | marc_columbia/Columbia-extract-20221130-009.mrc:46104515:2560 |
| Source | marc_columbia |
| Download Link | /show-records/marc_columbia/Columbia-extract-20221130-009.mrc:46104515:2560?format=raw |
LEADER: 02560cam a2200445 a 4500
001 4045504
005 20221027023839.0
008 930427t19941994nyua b 001 0 eng
010 $a 93013780
020 $a0716770148
035 $a(OCoLC)ocm28148592
035 $9(CU)AHU0980
035 $9AKF8418HS
035 $a(NNC)4045504
035 $a4045504
040 $aDLC$cDLC$dTMV$dAGL$dNNC-M
050 00 $aTP248.27.Y43$bY33 1994
070 0 $aTP248.27.Y43Y33$b1994
072 0 $aX500$aX600
082 00 $a589.2/330415/0724$220
245 00 $aYAC libraries :$ba user's guide /$cedited by David L. Nelson, Bernard H. Brownstein.
260 $aNew York :$bW.H. Freeman and Co.,$c[1994], ©1994.
300 $axx, 211 pages :$billustrations ;$c23 cm.
336 $atext$btxt$2rdacontent
337 $aunmediated$bn$2rdamedia
490 1 $aUWBC biotechnical resource series
504 $aIncludes bibliographical references and index.
505 0 $a1. Vectors and Host Strains for Cloning and Modification of Yeast Artificial Chromosomes -- 2. Yeast Spheroplast Transformation with Large DNA -- 3. Large Insert Yeast Artificial Chromosome Libraries -- 4. Isolation of YAC Clones from Genomic Libraries by Southern Blot Hybridization -- 5. Generation of YAC Contigs by Walking -- 6. The Use of YACs to Identify Expressed Sequences: cDNA Screening Using Total YAC Insert -- 7. YAC Clones as an Integral Component in the Positional Cloning of Human Disease Genes: Isolation of the APC Gene -- 8. Transfer of YAC DNA into Mammalian Cells and Transgenic Mice -- 9. Fluorescent in Situ Hybridization to Metaphase Chromosomes with YACs -- 10. YAC Breeding to Reconstitute Large Loci -- Appendix: Growth Media.
650 0 $aYeast fungi$xBiotechnology.$0http://id.loc.gov/authorities/subjects/sh87002576
650 0 $aYeast fungi$xGenetic engineering.$0http://id.loc.gov/authorities/subjects/sh89002537
650 0 $aChromosomes$vCatalogs.
650 2 $aBiotechnology.$0https://id.nlm.nih.gov/mesh/D001709
650 2 $aChromosomes, Fungal.$0https://id.nlm.nih.gov/mesh/D015825
650 2 $aGenetic Engineering.$0https://id.nlm.nih.gov/mesh/D005818
650 2 $aYeasts$xgenetics.$0https://id.nlm.nih.gov/mesh/D015003Q000235
655 2 $aCatalog.$0https://id.nlm.nih.gov/mesh/D019494
700 1 $aNelson, David L.,$d1956-$0http://id.loc.gov/authorities/names/n93039622
700 1 $aBrownstein, Bernard H.$0http://id.loc.gov/authorities/names/n93040184
830 0 $aUWBC biotechnical resource series.
852 00 $boff,hsl$hTP248.27.Y43$iY33 1994