MARC record from Internet Archive

LEADER: 05968cam 2200925 a 4500
001 ocm35574824
003 OCoLC
005 20200522015637.0
008 960802s1995 enka b 001 0 eng
010 $a 96176054
040 $aDLC$beng$cDLC$dEUN$dCSZ$dKKS$dAGL$dUKM$dORU$dIOG$dUBA$dBAKER$dNLGGC$dBTCTA$dYDXCP$dZWZ$dOCLCQ$dBDX$dOCLCF$dOCLCQ$dOCLCO$dTPH$dOCLCO$dOCL$dOCLCO$dOCLCA$dOCLCQ$dOCLCO$dVPI$dDGU$dCOF$dZAS$dOCLCO$dEQF$dLIP$dOCLCO$dTFH$dOCLCO$dVTM$dBUF$dOCLCO$dGZH$dOCLCO$dPAU$dOCLCO$dSNN$dOCLCO$dUWO$dOCLCO$dOCLCA$dOCLCQ$dGZW$dOCLCO$dOCLCA$dIEUOL$dOCLCA
015 $aGB9559848$2bnb
019 $a32688689$a33044352
020 $a0199634246$q(pbk.)
020 $a9780199634248$q(pbk.)
020 $a0199634254$q(hdb.)
020 $a9780199634255$q(hdb.)
035 $a(OCoLC)35574824$z(OCoLC)32688689$z(OCoLC)33044352
050 00 $aQP606.D46$bP34 1995
060 $aQH 450 P7833
070 0 $aQH450.3.P37$b1995
072 0 $aX300
082 00 $a574.87/328$220
084 $a42.20$2bcl
245 00 $aPCR 2 :$ba practical approach /$cedited by M.J. McPherson, B.D. Hames, and G.R. Taylor.
246 3 $aPCR two
264 1 $aOxford ;$aNew York :$bIRL Press at Oxford University Press,$c1995.
300 $axxv, 332 pages :$billustrations ;$c24 cm.
336 $atext$btxt$2rdacontent
337 $aunmediated$bn$2rdamedia
338 $avolume$bnc$2rdacarrier
490 1 $aThe practical approach series
500 $a"PCR 2 ... sets out to address different applications of PCR and so is complementary to the original volume"--Preface.
504 $aIncludes bibliographical references and index.
505 0 $aOptimizing PCR / K.K. Kidd and G. Ruano -- Use of specialty phosphoramidites in PCR / Michael J. McLean -- Chemical methods for 5' non-isotopic labelling of PCR probes and primers / Alex Andrus -- Solid phase PCR / Stefan Stamm and Jürgen Brosius -- Solid phase sequencing of PCR products / Johan Wahlberg, Thomas Hultman, and Mathias Uhlén -- cDNA cloning by RT-PCR / Jean Baptiste Dumas Milne Edwards [and others] -- Quantification of DNA and RNA by PCR / Jie Kang [and others] -- PCR MIMICs : competitive DNA fragments for use in quantitative PCR / Paul D. Siebert and David E. Kellogg -- In vitro expression of proteins from PCR products / Scott A. Lesley -- PCR-based approaches to human genome mapping / J.F.J. Morrison and A.F. Markham -- Fingerprinting of DNA and RNA using arbitrarily primed PCR / John Welsh [and others] -- Mutational analysis : known mutations / Clive R. Newton -- Mutational analysis : new mutations / K. Michaelides [and others] -- Linear amplification for the in vivo study of ligand/DNA interactions / Hans Peter Saluz and Jean-Pierre Jost -- Ligation-mediated PCR / Paul A. Garrity, Barbara Wold, and Paul R. Mueller.
520 1 $a"PCR is now an indispensable tool for the molecular biologist, heralding an era of unparalleled research productivity. The basic PCR technique provides the cornerstone of in vitro DNA amplification, upon which further features can be built to expand the range of PCR-based applications." "PCR 2: A Practical Approach is not a revised version of PCR: A Practical Approach, but sets out to address some of the exciting new applications of PCR and so it is complementary to the original volume. The book starts with an essential guide for improving PCRs, then deals with the use of modified primers and the direct sequencing of PCR products. The focus then switches to mRNA and covers cDNA cloning, mRNA quantitation, and direct expression of proteins from PCR products. Finally genomic DNA analysis is addressed including genome mapping and fingerprinting, the analysis of mutations, and the direct sequencing and analysis of methylation patterns. The book is essential reading for all researchers in molecular biology and related disciplines."--BOOK JACKET.
650 0 $aPolymerase chain reaction$vLaboratory manuals.
650 1 $aDNA Replication.
650 2 $aPolymerase Chain Reaction$xmethods.
650 2 $aNucleic Acids$xchemical synthesis.
650 2 $aNucleic Acids$xanalysis.
650 7 $aPolymerase chain reaction.$2fast$0(OCoLC)fst01070553
650 7 $aRéplication de l'ADN.$2fmesh
650 7 $aAmplification génique.$2fmesh
650 7 $aRéaction de polymérisation en chaîne.$2fmesh
650 7 $aRéaction de polymérisation en chaîne$xMéthodes.$2fmesh
650 17 $aPolymerase kettingreactie.$2gtt
650 7 $aBioquimica.$2larpcal
650 7 $aGenetica.$2larpcal
650 7 $aGénétique moléculaire$xTechnique.$2ram
650 7 $aAmplification génique.$2ram
650 7 $aRéaction en chaîne de la polymérase.$2ram
650 7 $aADN$xRéplication.$2ram
650 7 $aADN polymérases.$2ram
650 7 $aARN polymérases.$2ram
653 0 $aDNA$aSynthesis
655 7 $aLaboratory manuals.$2fast$0(OCoLC)fst01920776
655 7 $aLaboratory manuals.$2lcgft
700 1 $aMcPherson, M. J.
700 1 $aHames, B. D.
730 0 $aPCR, a practical approach.
830 0 $aPractical approach series.
856 41 $3Table of contents$uhttp://catdir.loc.gov/catdir/enhancements/fy0603/96176054-t.html
856 42 $3Publisher description$uhttp://catdir.loc.gov/catdir/enhancements/fy0603/96176054-d.html
938 $aBaker & Taylor$bBKTY$c95.00$d95.00$i0199634254$n0002625214$sactive
938 $aBaker & Taylor$bBKTY$c128.00$d128.00$i0199634246$n0002625209$sactive
938 $aBrodart$bBROD$n47802499$c$99.00
938 $aBaker and Taylor$bBTCP$n96176054
938 $aYBP Library Services$bYANK$n119047
029 1 $aAU@$b000011351364
029 1 $aAU@$b000023967970
029 1 $aAU@$b000024379131
029 1 $aHEBIS$b035635312
029 1 $aNLGGC$b138675090
029 1 $aNZ1$b4582170
029 1 $aYDXCP$b119046
029 1 $aYDXCP$b119047
029 1 $aAU@$b000060892957
994 $aZ0$bP4A
948 $hHELD BY P4A - 278 OTHER HOLDINGS