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LEADER: 06673cam 22007814a 4500
001 ocm39856415
003 OCoLC
005 20191227014602.0
008 981202s1998 njua b 001 0 eng
010 $a 98226406
040 $aDLC$beng$cDLC$dNLM$dMIA$dBAKER$dBTCTA$dYDXCP$dOCLCG$dIG#$dHEBIS$dDEBBG$dOCL$dZR1$dTULIB$dSGE$dILU$dOCLCF$dOCLCO$dOCLCQ$dOCLCO$dOCLCQ$dOCLCO$dCASUM$dOCLCO$dOCLCA$dCPO$dOCLCO$dOCLCA$dOCLCQ$dOCLCA
016 7 $a9814781$2DNLM
019 $a1027340956
020 $a0896035018$q(alk. paper)
020 $a9780896035010$q(alk. paper)
020 $a0896034399
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035 $a(OCoLC)39856415$z(OCoLC)1027340956
042 $apcc
050 00 $aQH506$b.M6642 1998
060 00 $aQZ 50$bM71832 1998
082 00 $a572.8$221
084 $aWC 1200$2rvk
084 $aWC 4150$2rvk
084 $aWC 4460$2rvk
084 $aBIO 220f$2stub
084 $aBIO 180f$2stub
084 $aCHE 860f$2stub
245 00 $aMolecular biomethods handbook /$cedited by Ralph Rapley and John M. Walker.
260 $aTotowa, N.J. :$bHumana Press,$c℗♭1998.
300 $axiii, 725 pages :$billustrations ;$c26 cm
336 $atext$btxt$2rdacontent
337 $aunmediated$bn$2rdamedia
338 $avolume$bnc$2rdacarrier
504 $aIncludes bibliographical references and index.
505 0 $aExtraction of total RNA from tissues and cultured cells / Sandeep Raha, Mingfu Ling and Frank Merante -- Isolation of total cellular DNA from tissues and cultured cells / Frank Merante, Sandeep Raha, and Mingfu Ling -- Gel electrophoresis of DNA / Duncan R. Smith -- S1 nuclease protection assay (RPA) / Ralf Einspanier and Annette Plath -- Gene Probes / Marilena Aquino de Muro -- Nonradioactive labeling of DNA / Thomas P. McCreery and Terrance R. Barrette -- Southern blot analysis / Paolo A. Sabelli -- Northern blot analysis / Paolo A. Sabelli -- DNA sequencing / Chris Spencer -- Autoradiography and fluorography / Bronwen Harvey -- Mobility shift assays / Nigel J. Savery and Stephen J.W. Busby -- cDNA libraries / Ian G. Cowell -- Genomic DNA libraries / Bimal D.M. Theophilus -- [lambda] as a cloning vector / Rupert Mutzel -- Plasmid-derived cloning vectors / Craig Winstanley and Ralph Rapley -- M13 and phagemid-based cloning vectors / Ralph Rapley -- Retroviral vectors : from laboratory tools to moleculare medicines / Richard G. Vile, Anna Tuszynski and Simon Castledon -- Baculovirus vectors / Azeem Ansari and Vincent C. Emery.
505 0 $aGene transfer and expression in tissue culture cells of higher eukaryotes / M. Alexandra Aitken, Selina Raguz and Michael Antoniou -- Plant transformation / Andy Prescott, Rob Briddon and Wendy Harwood -- Restriction fragment-length ploymorphisms / Elaine K. Green -- Genome mapping / Jacqueline Boultwood -- Yeast artificial chromosomes / Angela Flannery and Rakesh Anand -- Polymerase chain reaction / Ralph Rapley -- In vitro transcription / Martin J. Tymms -- Site-directed mutagenesis / John R. Adair and T. Paul Wallace -- Transgenic techniques / Roberta M. James and Paul Dickinson -- DNA profiling : theory and practice / Karen M. Sullivan -- Radiolabeling of peptides and proteins / Arvind C. Patel and Stewart R. Mattewson -- Protein electrophoresis / David J. Begley -- Protein blotting : principles and applications / Peter R. Shewry and Roger J. Fido -- Ion-exchange chromatography / Paul Cutler -- Hydrophobic interaction chromatography / Paul A. O'Farrell -- Affinity chromatography / George W. Jack -- Reversed-phase HPLC / Bill Neville -- Glycoprotein analysis / Terry D. Butters -- Protein sequencing / Bryan J. Smith and John R. Chapman -- Solid-phase peptide synthesis / Gregg B. Fields -- Protein engineering / Sudhir Paul -- Monoclonal antibodies / Chritopher Dean and Helmout Modjtahedi -- Phage-display libraries / Julie E. Thompson and Andrew J. Williams -- Enzyme-linked immunosorbent assay (ELISA) / John R. Crowther -- Epitope mapping : identification of antibody binding sites on protein antigens / Glenn E. Morris -- Immunocytochemistry / Lorette C. Javois -- Flow cytometry / Robert E. Cunningham -- Mass spectrometry / John R. Chapman -- The technique of In situ hybridization : principles and applications / Desire du Sart and K.H. Andy Choo.
520 1 $a"In the Molecular Biomethods Handbook, Ralph Rapley and John Walker assemble an authoritative team of investigators to illuminate the core bioanalytical techniques used every day in their own laboratories, and laboratories throughout the world. These highly experienced writers fully explain both the theory behind, and the application of, these key techniques, and include extensive references for those seeking detailed laboratory protocols. The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few.
520 8 $aNumerous key protein methods, as well as support and related techniques, are also included."--Jacket.
650 0 $aMolecular biology$xMethodology.
650 12 $aGenetic Techniques.
650 22 $aCytological Techniques.
650 22 $aMolecular Biology.
650 7 $aMolecular biology$xMethodology.$2fast$0(OCoLC)fst01024745
650 7 $aMethode$2gnd
650 7 $aMolekularbiologie$2gnd
650 7 $aAnalytische Methode$2gnd
650 7 $aBiochemie$2gnd
655 4 $aFulltext.
655 4 $aInternet Resources.
700 1 $aRapley, Ralph.
700 1 $aWalker, John M.,$d1948-
856 41 $3Table of contents$uhttp://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=007838888&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
856 41 $3Table of contents$uhttp://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007838888&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
856 41 $uhttps://link.springer.com/book/10.1007/978-1-59259-642-3$zAccess restricted to Stanford Community
856 4 $uhttp://www.springerprotocols.com/BookToc/doi/10.1007/978-1-59259-642-3
938 $aBaker & Taylor$bBKTY$c110.00$d110.00$i0896035018$n0003164341$sactive
938 $aBaker and Taylor$bBTCP$n98226406
938 $aIngram$bINGR$n9780896035010
938 $aYBP Library Services$bYANK$n1379812
029 1 $aAU@$b000014122795
029 1 $aDEBBG$bBV011632695
029 1 $aHEBIS$b090947762
029 1 $aIG#$b9780896035010
029 1 $aNLM$b9814781
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994 $aZ0$bP4A
948 $hNO HOLDINGS IN P4A - 296 OTHER HOLDINGS