MARC record from Internet Archive

LEADER: 07086cam 22012134a 4500
001 ocm50725188
003 OCoLC
005 20221010224931.0
008 020923s2003 nyua b 001 0 eng
010 $a 2002034976
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060 00 $a2003 B-920
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245 00 $aManipulating the mouse embryo :$ba laboratory manual /$cAndras Nagy [and others].
250 $a3rd ed.
260 $aCold Spring Harbor, N.Y. :$bCold Spring Harbor Laboratory Press,$c©2003.
300 $ax, 764 pages :$billustrations (some color) ;$c29 cm
336 $atext$btxt$2rdacontent
337 $aunmediated$bn$2rdamedia
338 $avolume$bnc$2rdacarrier
504 $aIncludes bibliographical references and index.
520 $aProvides background information and detailed protocols for developing a mouse colony and using the animals in transgenic and gene-targeting experiments. The protocols list the animals, equipment, and reagents required and step-by-step procedures. Topics include in vitro culture of preimplantation embryos, surgical procedures, the production of chimeras, and the analysis of genome alterations. The third edition adds protocols for cloning mice, modifying embryonic stem cells, intracytoplasmic sperm injection, and cryopreservation of embryos.
505 00 $g1.$tDevelopmental Genetics and Embryology of the Mouse: Past, Present, and Future --$g2.$tSummary of Mouse Development --$g3.$tProduction of Transgenic and Chimeric Mice: General Issues --$g4.$tRecovery and In Vitro Culture of Preimplantation-stage Embryos --$g5.$tIsolation, Culture, and Manipulation of Postimplantation Embryos --$g6.$tSurgical Procedures --$g7.$tProduction of Transgenic Mice --$g8.$tIsolation and Culture of Blastocyst-derived Stem Cell Lines --$g9.$tVector Designs for Embryonic Stem Cell-based Transgenesis and Genome Alterations --$g10.$tIntroduction of Foreign DNA into Embryonic Stem Cells --$g11.$tProduction of Chimeras --$g12.$tDetection and Analysis of Mouse Genome Alterations and Specific Sequences --$g13.$tParthenogenesis, Pronuclear Transfer, and Mouse Cloning --$g14.$tAssisted Reproduction: Ovary Transplantation, In Vitro Fertilization, Artificial Insemination, and Intracytoplasmic Sperm Injection --$g15.$tCryopreservation, Rederivation, and Transport of Mice --$g16.$tTechniques for Visualizing Gene Products, Cells, Tissues, and Organ Systems --$g17.$tSetting Up a Micromanipulation Lab --$gAppendix 1.$tBuffers and Solutions --$gAppendix 2.$tCautions --$gAppendix 3.$tSuppliers.
650 0 $aMice$xEmbryos$vLaboratory manuals.
650 0 $aMice$xGenetic engineering$vLaboratory manuals.
650 0 $aTransgenic mice$vLaboratory manuals.
650 0 $aMice as laboratory animals.
650 0 $aGenetic engineering.
650 12 $aGenetic Engineering
650 12 $aMice$xembryology
650 22 $aMice, Transgenic$xgenetics
650 6 $aSouris$xEmbryons$vManuels de laboratoire.
650 6 $aSouris$xGénie génétique$vManuels de laboratoire.
650 6 $aSouris transgéniques$vManuels de laboratoire.
650 6 $aSouris (Animal de laboratoire)
650 6 $aGénie génétique.
650 7 $aGenetic engineering.$2fast$0(OCoLC)fst00940027
650 7 $aMice as laboratory animals.$2fast$0(OCoLC)fst01019388
650 7 $aMice$xEmbryos.$2fast$0(OCoLC)fst01019350
650 7 $aMice$xGenetic engineering.$2fast$0(OCoLC)fst01019358
650 7 $aTransgenic mice.$2fast$0(OCoLC)fst01154669
650 7 $aEmbryo$2gnd
650 7 $aEmbryonalentwicklung$2gnd
650 7 $aExperiment$2gnd
650 7 $aMaus$2gnd
650 7 $aTransgene Tiere$2gnd
650 7 $aGentechnologie$2gnd
650 7 $aLabortechnik$2gnd
650 7 $aRatos (embriologia)$2larpcal
650 7 $aAnimais de laboratório.$2larpcal
650 7 $aEmbriologia comparada.$2larpcal
650 07 $aEmbriologia$vManuals de laboratori.$2lemac
650 07 $aMaus.$2swd
655 2 $aLaboratory Manual
655 7 $aLaboratory manuals.$2fast$0(OCoLC)fst01920776
655 7 $aLaboratory manuals.$2lcgft
655 7 $aManuels de laboratoire.$2rvmgf
700 1 $aNagy, Andras,$d1951-
776 08 $iOnline version:$tManipulating the mouse embryo.$b3rd ed.$dCold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, ©2003$w(OCoLC)606912042
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