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LEADER: 06137cam 2200997Ia 4500
001 ocn666954936
003 OCoLC
005 20200514022812.0
008 100929s2000 enka ob 001 0 eng d
006 m o d
007 cr bn|||||||||
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019 $a53957536
020 $a9780585483955$q(electronic bk.)
020 $a0585483957$q(electronic bk.)
020 $z9780199637935$q(hbk.)
020 $z0199637938$q(hbk.)
020 $z9780199637928$q(pbk.)
020 $z019963792X$q(pbk.)
035 $a(OCoLC)666954936$z(OCoLC)53957536
050 4 $aQH442.3$b.G456 2000
060 4 $a2000 I-069
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245 00 $aGene targeting :$ba practical approach /$cedited by Alexandra L. Joyner.
250 $a2nd ed.
260 $aOxford ;$aNew York :$bOxford University Press,$c2000.
300 $a1 online resource (xviii, 293 pages) :$billustrations.
336 $atext$btxt$2rdacontent
337 $acomputer$bc$2rdamedia
338 $aonline resource$bcr$2rdacarrier
490 1 $aPractical approach series
504 $aIncludes bibliographical references and index.
588 0 $aPrint version record.
520 $a"This new edition of Gene Targeting: A Practical Approach contains a remarkable collection of state-of-the-art protocols as well as thoughtful discussion of how to design a new mouse mutation and analyse the resulting phenotype to maximise the information gained. The techniques described for gene targeting in mouse ES cells, are equally applicable for gene targeting in other tissue culture cells, including human cells. The second edition describes additional genetic approaches for studying gene function in mice, including chimera analysis, gene trap (GT) approaches and powerful ways to combine gene targeting with classical genetics." "Gene Targeting: A Practical Approach (second edition) is a practical tool and information resource for scientists working on fundamental or applied aspects of gene targeting, ES cell manipulation, and analysis of mouse mutants. It will be invaluable to all researchers who wish to use the approaches for studies of gene function in cells or mice."--Provided by publisher.
505 0 $aGene targeting, principles, and practice in mammalian cells / Paul Hasty, Alejandro Abuin, and Allan Bradley -- Site-specific recombination in cells and mice / Susan M. Dymecki -- Production of targeted embryonic stem cell clones / Michael P. Matise, Wojtek Auerbach and Alexandra L. Joyner -- Production of chimeras by blastocyst and morula injection of targeted ES cells / Virginia Papaioannou and Randall Johnson -- Production and analysis of ES cell aggregation chimeras / Andreas Nagy and Janet Rossant -- Gene trap strategies in ES cells / Wolfgang Wurst and Achim Gossler -- Classical genetics and gene targeting / Scott Bultman and Terry Magnuson.
650 0 $aGene targeting$vLaboratory manuals.
650 0 $aGene targeting.
650 7 $aSCIENCE$xBiotechnology.$2bisacsh
650 7 $aCiblage ge ne.$2fmesh
650 7 $aGene targeting.$2fast$0(OCoLC)fst00939637
650 17 $aGenetische manipulatie.$2gtt
650 17 $aMethoden.$2gtt
650 7 $aCiblage d'un ge ne$xManuels de laboratoire.$2ram
650 7 $aBlutstammzelle$2gnd
650 7 $aGen-Targeting$2gnd
650 7 $aGentechnologie$2gnd
650 07 $aMaus.$2swd
650 7 $aMethode$2gnd
650 2 $aGene Targeting.
655 4 $aElectronic books.
655 7 $aLaboratory manuals.$2fast$0(OCoLC)fst01920776
655 2 $aLaboratory Manual.
700 1 $aJoyner, Alexandra L.,$eeditor.
776 08 $iPrint version:$tGene targeting.$b2nd ed.$dOxford ; New York : Oxford University Press, 2000$z9780199637935$z9780199637928$w(DLC) 99036660$w(OCoLC)41649667
830 0 $aPractical approach series.
856 42 $3Table of contents$uhttp://catdir.loc.gov/catdir/enhancements/fy0606/99036660-t.html
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856 42 $3Publisher description$uhttp://catdir.loc.gov/catdir/enhancements/fy0606/99036660-d.html
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