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LEADER: 08494cam 2200925Ma 4500
001 ocm50175109
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008 020618s2002 njua ob 001 0 eng d
006 m o d
007 cr cn|||||||||
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020 $a0585405875$q(electronic bk.)
020 $a9780585405872$q(electronic bk.)
020 $a1592592414
020 $a9781592592418
020 $z0896038815$q(alk. paper)
020 $z9780896038813$q(alk. paper)
035 $a(OCoLC)50175109$z(OCoLC)81161471$z(OCoLC)123113531$z(OCoLC)230743793$z(OCoLC)245541363$z(OCoLC)320965250$z(OCoLC)533449406$z(OCoLC)642460501$z(OCoLC)702098386$z(OCoLC)771203349$z(OCoLC)961672297$z(OCoLC)962633235$z(OCoLC)964921200$z(OCoLC)967626907$z(OCoLC)984879305$z(OCoLC)991984015$z(OCoLC)994818756$z(OCoLC)999440427$z(OCoLC)1005820531$z(OCoLC)1007378106$z(OCoLC)1053041962$z(OCoLC)1069512230$z(OCoLC)1077960735$z(OCoLC)1081183655$z(OCoLC)1086922371$z(OCoLC)1097327891$z(OCoLC)1135415618
050 4 $aQH440.5$b.E43 2002eb
060 4 $aW1$bME9616J v.185 2002
060 4 $aQU 25$bE53 2002
072 7 $aMED$x075000$2bisacsh
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245 00 $aEmbryonic stem cells :$bmethods and protocols /$cedited by Kursad Turksen.
260 $aTotowa, N.J. :$bHumana Press,$c2002.
300 $a1 online resource (xvi, 499 pages) :$billustrations (some color)
336 $atext$btxt$2rdacontent
337 $acomputer$bc$2rdamedia
338 $aonline resource$bcr$2rdacarrier
490 1 $aMethods in molecular biology ;$v185
504 $aIncludes bibliographical references and index.
588 0 $aPrint version record.
520 8 $aAnnotation$bKursad Turksen and a panel of international experts describe their most productive methods for using embryonic stem (ES) cells as in vitro developmental models for many cell and tissue types. Set out in step-by-step detail, these protocols range widely from ES cell isolation, maintenance, and modulation of gene expression, to cutting-edge techniques that use cDNA arrays in gene expression analysis and phage display libraries, the generation of antibodies against very rare antigens, and the identification of and characterization of protein and protein interactions.
505 0 $a""Embryonic Stem Cells""; ""Preface""; ""Contents""; ""Contributors""; ""Color Plates""; ""1 Methods for the Isolation and Maintenance of Murine Embryonic Stem Cells""; ""2 The Use of a Chemically Defi ned Media for the Analyses of Early Development in ES Cells and Mouse Embryos""; ""3 Analysis of the Cell Cycle in Mouse Embryonic Stem Cells""; ""4 Murine Embryonic Stem Cells as a Model for Stress Proteins and Apoptosis During Differentiation""; ""5 Effects of Altered Gene Expression on ES Cell Differentiation""; ""6 Hypoxic Gene Regulation in Differentiating ES Cells""
505 8 $a""7 Regulation of Gap Junction Protein (Connexin) Genes and Function in Differentiating ES Cells""""8 Embryonic Stem Cell Differentiation as a Model to Study Hematopoietic and Endothelial Cell Development""; ""9 Analysis of Bcr-Abl Function Using an In Vitro Embryonic Stem Cell Differentiation System""; ""10 Embryonic Stem Cells as a Model for Studying Osteoclast Lineage Development""; ""11 Differentiation of Embryonic Stem Cells as a Model to Study Gene Function During the Development of Adipose Cells""; ""12 Embryonic Stem Cell Differentiation and the Vascular Lineage""
505 8 $a""13 Embryonic Stem Cells as a Model to Study Cardiac, Skeletal Muscle, and Vascular Smooth Muscle Cell Differentiation""""14 Cardiomyocyte Enrichment in Differentiating ES Cell Cultures: Strategies and Applications""; ""15 Embryonic Stem Cells as a Model for the Physiological Analysis of the Cardiovascular System""; ""16 Isolation of Lineage-Restricted Neural Precursors from Cultured ES Cells""; ""17 Lineage Selection for Generation and Amplifi cation of Neural Precursor Cells""
505 8 $a""18 Selective Neural Induction from ES Cells by Stromal Cell-Derived Inducing Activity and Its Potential Therapeutic Application in Parkinson�s Disease""""19 Epidermal Lineage""; ""20 ES Cell Differentiation Into the Hair Follicle Lineage In Vitro""; ""21 Embryonic Stem Cells as a Model for Studying Melanocyte Development""; ""22 Using Progenitor Cells and Gene Chips to Defi ne Genetic Pathways""; ""23 ES Cell-Mediated Conditional Transgenesis""; ""24 Switching on Lineage Tracers Using Site-Specifi c Recombination""; ""25 From ES Cells to Mice: The Gene Trap Approach""
505 8 $a""26 Functional Genomics by Gene-Trapping in Embryonic Stem Cells""""27 Phage-Displayed Antibodies to Detect Cell Markers""; ""28 Gene Transfer Using Targeted Filamentous Bacteriophage""; ""29 Single-Cell PCR Methods for Studying Stem Cells and Progenitors""; ""30 Nonradioactive Labeling and Detection of mRNAs Hybridized onto Nucleic Acid cDNA Arrays""; ""31 Expression Profiling Using Quantitative Hybridization on Macroarrays""; ""32 Isolation of Antigen-Specifi c Intracellular Antibody Fragments as Single Chain Fv for Use in Mammalian Cells""
650 0 $aEmbryonic stem cells$vLaboratory manuals.
650 12 $aStem Cells
650 7 $aMEDICAL$xPhysiology.$2bisacsh
650 7 $aSCIENCE$xLife Sciences$xHuman Anatomy & Physiology.$2bisacsh
650 7 $aEmbryonic stem cells.$2fast$0(OCoLC)fst00908443
655 2 $aLaboratory Manuals
655 2 $aLaboratory Manual.
655 4 $aElectronic books.
655 7 $aLaboratory manuals.$2fast$0(OCoLC)fst01920776
700 1 $aTurksen, Kursad.
773 0 $tSpringer Protocols
776 08 $iPrint version:$tEmbryonic stem cells.$dTotowa, N.J. : Humana Press, 2002$z0896038815$w(DLC) 2001026459
830 0 $aMethods in molecular biology (Clifton, N.J.) ;$v185.
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