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RNA methodologies
Robert E. Farrell

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RNA methodologies
laboratory guide for isolation and characterization
4th ed.
Robert E. Farrell Jr

Published 2010 by Elsevier/Academic Press in Amsterdam, Boston .
Written in English.

About the Book

This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize time at the bench. The abundant use of flow charts, tables and graphs are especially helpful in the planning and implementation phases of a project and facilitate learning. 30% new material in this edition includes the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary.

Table of Contents

CHAPTER 1: RNA and the Cellular Biochemistry Revisited
CHAPTER 2: RNA Isolation Strategies
CHAPTER 3: The Truth about Tissues
CHAPTER 4: Going Green: RNA and the Molecular Biology of Plants (NEW)
CHAPTER 5: Isolation of Polyadenylated RNA
CHAPTER 6: Quality Control for RNA Preparations (EXPANDED)
CHAPTER 7: Resilient Ribonucleases
CHAPTER 8: Stringency: Conditions that Influence Nucleic Acid Structure (EXPANDED)
CHAPTER 9: Electrophoresis of RNA (EXPANDED)
CHAPTER 10: Photodocumentation and Image Analysis (EXPANDED)
CHAPTER 11: Northern Analysis
CHAPTER 12: Nucleic Acid Probe Technology
CHAPTER 13: Practical Nucleic Acid Hybridization
CHAPTER 14: Principles of Detection (EXPANDED)
CHAPTER 15: Quantification of Specific mRNAs by Nuclease Protection
CHAPTER 16: Analysis of Nuclear RNA (EXPANDED)
CHAPTER 19: Quantitative PCR Techniques
CHAPTER 20: Functional Genomics and Transcript Profiling (NEW)
CHAPTER 21: High-Throughput Analysis of Gene Expression
CHAPTER 22: Non-Array Methods for Global Analysis of Gene Expression (NEW)
CHAPTER 23: RNA Interference: Targeted Gene Silencing (EXPANDED)
CHAPTER 24: Genomes, Transcriptomes, Proteomes, and Bioinformatics
CHAPTER 25: An RNA Paradigm.

Edition Notes

Previous ed.: 2005.

Includes bibliographical references and index.

Electronic reproduction. Amsterdam : Elsevier Science & Technology, 2010. Mode of access: World Wide Web. System requirements: Web browser. Title from title screen (viewed on Feb. 24, 2010). Access may be restricted to users at subscribing institutions.


Library of Congress
QP623 .F37 2010

The Physical Object

[electronic resource] :
xxiii, 717 p. :
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