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January 29, 2010 | History

Practical biotransformations 1 edition

Practical biotransformations
Gideon Grogan

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Practical biotransformations
a beginner's guide
Gideon Grogan.

Published 2009 by Wiley in Chichester, West Sussex .
Written in English.

Table of Contents

Biotransformations, microbes and enzymes
Introduction
Biotransformations
Microorganisms
Organism nomenclature
Enzymes
Types of enzymatic reactions
Enzymatic cofactors
Some basic characteristics of enzyme catalysis
Types of biocatalyst biotransformations by whole cells or isolated enzymes
Conclusion
An overview of biocatalyst sources and web-based information
Introduction
Microbial culture collections
Obtaining organisms from other research groups
Selective enrichments
Metagenomics
Enzyme suppliers and biocatalyst development companies
Genome mining for biocatalysts
Obtaining amino acid and gene sequence information on biocatalysts
Obtaining DNA templates for cloning
Custom gene synthesis
Other interesting web resources for biocatalysis
Conclusion
Setting up a laboratory for biotransformations
Introduction
Microbiological containment
On containment issues and genetically-modified organisms
Equipment for handling microorganisms
Techniques and terms in microbiology sterility, asepsis and aseptic technique
Disposal of viable microbial waste and disinfection of reusable equipment
Equipment for enzymology and molecular biology
General reagents and chemicals in a biotransformations laboratory
Conclusion
A beginner's guide to preparative whole-cell microbial biotransformations
Introduction
Storage, maintenance and growth of microorganisms
General microbiological methods
Examples of whole-cell biotransformations using bacteria
Biotransformation by filamentous fungi and yeasts
Whole-cell biotransformations by recombinant strains of E. coli
Conclusion
A beginner's guide to biotransformations by commercially available isolated enzymes
Introduction
Lipases
Hydrolytic reactions using lipases
Using lipases for acylation reactions
Other hydrolases
Commercially available coenzyme-dependent enzymes
Carbon-carbon bond forming reactions
Conclusion
A beginner's guide to the isolation and analysis and use of home-grown enzyme biocatalysts
Introduction
Cell growth and harvesting
Cell disruption
A typical procedure for making a cell extract from a recombinant strain of E. coli
Purification of enzymes - a brief guide
Techniques for protein purification
Isolation of recombinant enzymes using histidine tags
Estimation of protein.

Edition Notes

Includes bibliographical references and index.

Series
Postgraduate chemistry series

Classifications

Dewey Decimal Class
660.6
Library of Congress
QD415.5 .G76 2009

The Physical Object

Pagination
p. cm.

ID Numbers

Open Library
OL23057270M
Internet Archive
practicalbiotran00grog
ISBN 13
9781405193672, 9781405171250
LC Control Number
2009004187
Goodreads
7021796 7021795

History

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January 29, 2010 Edited by WorkBot add more information to works
December 11, 2009 Created by WorkBot add works page