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January 24, 2010 | History

Experimental validation of a microfluidic real time PCR device and the development of microheater applications 1 edition

Experimental validation of a microfluidic real time PCR device and the ...
Rachel Fu

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Experimental validation of a microfluidic real time PCR device and the development of microheater applications.

Published 2005 .
Written in English.

About the Book

Microfluidics is the study of small-scale transport phenomena and has great potential in the field of biomedical research. Lab-on-chip technology comprises microfluidic networks that allow for integration of numerous bioanalytical processes, offering many advantages over conventional systems. This thesis details research projects in three different areas of microfluidics. The first section describes the development and experimental validation of a miniaturized real time PCR (polymerase chain reaction) system, including DNA quantification results for Escherichia coli. The second section describes the design of an embedded microheater for poly(dimethylsiloxane) or PDMS microchannel systems. The thermal field generated by the microheater was measured using temperature dependent dye. The third section describes a novel method of flow velocity measurement in microchannels, by applying a heat pulse to temperature dependent dye flowing in a capillary. The heat pulse was tracked by monitoring fluorescent intensity, and the average flow velocity was determined from the heat pulse velocity.

Edition Notes

Source: Masters Abstracts International, Volume: 44-02, page: 1022.

Thesis (M.A.Sc.)--University of Toronto, 2005.

Electronic version licensed for access by U. of T. users.

GERSTEIN MICROTEXT copy on microfiche (2 microfiches).

The Physical Object

Pagination
90 leaves.
Number of pages
90

ID Numbers

Open Library
OL19217372M
ISBN 10
0494074078

History Created December 11, 2009 · 2 revisions Download catalog record: RDF / JSON

January 24, 2010 Edited by WorkBot add more information to works
December 11, 2009 Created by WorkBot add works page