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January 24, 2010 | History

Identification of a novel stem cell specific candidate silencer element in retrovirus vectors 1 edition

Identification of a novel stem cell specific candidate silencer elemen ...
Jason Chung-Sheung Ho

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Identification of a novel stem cell specific candidate silencer element in retrovirus vectors.

Published 2005 .
Written in English.

About the Book

The silencing of retrovirus vectors in stem cells poses a problem for their use in gene therapy and for studying stem cell biology. The identification of retrovirus silencer elements has led to the development of vectors with improved expression but these vectors are still susceptible to silencing in stem cells. DNase I footprinting across the entire MSCV retrovirus vector was performed, comparing F9 and NIH3T3 cell nuclear extracts. The results revealed differences in footprints and hypersensitive bases within the essential U5 and psi regions. Electrophoretic mobility shift assays were carried out to confirm binding and to identify the consensus sites through mutational analysis. Functional characterization of the U5 and psi regions using an episome repression assay identified a novel stem cell specific potential silencer element within the psi region.

Edition Notes

Source: Masters Abstracts International, Volume: 44-02, page: 0754.

Thesis (M.Sc.)--University of Toronto, 2005.

Electronic version licensed for access by U. of T. users.

GERSTEIN MICROTEXT copy on microfiche (1 microfiche).

The Physical Object

Pagination
71 leaves.
Number of pages
71

ID Numbers

Open Library
OL19217312M
ISBN 10
0494073942

History Created December 11, 2009 · 2 revisions Download catalog record: RDF / JSON

January 24, 2010 Edited by WorkBot add more information to works
December 11, 2009 Created by WorkBot add works page