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January 24, 2010 | History

Molecular mechanisms underlying the expression of proglucagon gene 1 edition

Molecular mechanisms underlying the expression of proglucagon gene
Shamim Lotfi

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Molecular mechanisms underlying the expression of proglucagon gene
role of the Epac signaling pathway in the intestinal endocrine L cells.

Published 2005 .
Written in English.

About the Book

Numerous reports have indicated that protein kinase A (PKA) is not the sole target of the second messenger cAMP. Although proglucagon gene expression and the synthesis of proglucagon encoded hormones could be activated by PKA activators such as Forskolin, whether the activation is entirely attributed to PKA has not been examined. We found that Forskolin also activates ERK1/2 phosphorylation in two intestinal proglucagon producing cell lines. The MEK inhibitors were found to repress the expression of proglucagon promoter as well as endogenous proglucagon mRNA in these cell lines, and to attenuate the stimulatory effect of Forskolin on proglucagon gene transcription. The Epac-pathway-specific cAMP analogue, 8pMeOPT-2'-O-Me-cAMP, effectively stimulated ERK1/2 phosphorylation as well as proglucagon mRNA expression and moderately stimulated proglucagon promoter expression. Finally, dominant negative (DN) Epac2 repressed Forskolin activated proglucagon promoter activity. We, therefore, suggest that cAMP regulates proglucagon expression, at least partially, via the Epac-Ras/Rap-MEK-ERK signaling pathway.

Edition Notes

Source: Masters Abstracts International, Volume: 44-02, page: 0763.

Thesis (M.Sc.)--University of Toronto, 2005.

Electronic version licensed for access by U. of T. users.

GERSTEIN MICROTEXT copy on microfiche (2 microfiches).

The Physical Object

121 leaves.
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History Created December 11, 2009 · 2 revisions Download catalog record: RDF / JSON

January 24, 2010 Edited by WorkBot add more information to works
December 11, 2009 Created by WorkBot add works page