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January 24, 2010 | History

The influence of mineral substrates and biological surroundings on the enrichment and isolation of pentachlorophenol-degrading bacteria from uncontaminated soils 1 edition

The influence of mineral substrates and biological surroundings on the ...
Liliana Gamboa

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The influence of mineral substrates and biological surroundings on the enrichment and isolation of pentachlorophenol-degrading bacteria from uncontaminated soils.

Published 2005 .
Written in English.

About the Book

The ability of uncontaminated soils to degrade pentachlorophenol (PCP), a priority pollutant, was determined. Uncontaminated soils showed a wide range of degradation rates without any obvious direct relation to specific vegetation. They were comparable to previously reported rates at pristine and even contaminated sites. PCP amended community structures were studied using 16S rDNA genes and analyzed by denaturing gradient gel electrophoresis (DGGE). A DNA-stable isotope probing (SIP) technique was applied both in the lab and in situ. Different PCP-degrading communities were enriched on different physical substrates. Sand proved to be the best media for transfer of PCP degraders while peat moss was the poorest. The use of novel baiting cores was successful in capturing a microbial community that gave mineralization capabilities consistent with that of the surrounding soil. This study provides evidence that a broad diversity of microorganisms is involved in PCP degradation in pristine soils.

Edition Notes

Source: Masters Abstracts International, Volume: 44-02, page: 0756.

Advisor: Roberta R. Fulthorpe

Thesis (M.Sc.)--University of Toronto, 2005.

Electronic version licensed for access by U. of T. users.

GERSTEIN MICROTEXT copy on microfiche (2 microfiches).

The Physical Object

Pagination
95 leaves.
Number of pages
95

ID Numbers

Open Library
OL19216228M
ISBN 10
0494071192

History

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January 24, 2010 Edited by WorkBot add more information to works
December 11, 2009 Created by WorkBot add works page