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January 24, 2010 | History

Gene delivery to human sweat glands: A model for cystic fibrosis gene therapy 1 edition

Gene delivery to human sweat glands: A model for cystic fibrosis gene ...
Benjamin Haeyul Lee

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Gene delivery to human sweat glands: A model for cystic fibrosis gene therapy.

Published 2006 .
Written in English.

About the Book

We investigated the possibility of using human sweat glands as a model for testing cystic fibrosis (CF) gene therapy vectors. Human sweat glands are easy to obtain from skin biopsy, and could provide a safe model to study the efficacy of CF gene therapy. Various ex vivo gene delivery methods were examined using helper-dependent adenovirus vectors. We found that transduction efficiency can be enhanced by pre-treating isolated sweat glands with dispase, suggesting that the basement membrane is a critical barrier to adenoviral vectors. Using this approach, we showed that CFTR could be efficiently delivered to, and expressed by the epithelial cells of sweat glands with our helper dependent adenoviral vector containing cytokeratin 18 regulatory elements. Based on this study we propose that sweat glands might be used as an alternative model to study CF gene therapy in humans.

Edition Notes

Source: Masters Abstracts International, Volume: 44-06, page: 2700.

Thesis (M.Sc.)--University of Toronto, 2006.

Electronic version licensed for access by U. of T. users.

ROBARTS MICROTEXT copy on microfiche.

The Physical Object

78 leaves.
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History Created December 11, 2009 · 2 revisions Download catalog record: RDF / JSON

January 24, 2010 Edited by WorkBot add more information to works
December 11, 2009 Created by WorkBot add works page