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January 24, 2010 | History

Gene CATCHR: Gene C&barbelow;loning a&barbelow;nd T&barbelow;agging for C&barbelow;aenorhabditis elegans using yeast H&barbelow;omologous R&barbelow;ecombination: A novel approach for the analysis of gene expression 1 edition

Gene CATCHR: Gene C&barbelow;loning a&barbelow;nd T&barbelow;agging fo ...
Holly Elizabeth Sassi

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Gene CATCHR: Gene C&barbelow;loning a&barbelow;nd T&barbelow;agging for C&barbelow;aenorhabditis elegans using yeast H&barbelow;omologous R&barbelow;ecombination: A novel approach for the analysis of gene expression.

Published 2006 .
Written in English.

About the Book

Expression patterns of gene products provide important insights into gene function. Reporter constructs are frequently used to study gene expression in Caenorhabditis elegans, but the sequence context of a given gene is inevitably altered in such constructs, which may therefore lack important regulatory elements. I describe the development of Gene C ATCHR, a novel method of generating reporter-fusions of worm genes that exploits yeast homologous recombination. Yeast homologous recombination facilitates the cloning of large genomic regions, allowing the isolation of complete open reading frames and regulatory sequences in promoters, untranslated regions and flanking DNA. Gene CATCHR thus preserves the original regulatory context of a given gene as much as possible. Comparisons to genes with known expression patterns validate the use of the Gene CATCHR system. Further, novel expression data was generated. Mutant rescue experiments demonstrate that Gene CATCHR-generated transgenes are functional. Gene CATCHR represents a valuable tool to study gene expression.

Edition Notes

Source: Masters Abstracts International, Volume: 44-06, page: 2712.

Thesis (M.Sc.)--University of Toronto, 2006.

Electronic version licensed for access by U. of T. users.

ROBARTS MICROTEXT copy on microfiche.

The Physical Object

Pagination
143 leaves.
Number of pages
143

ID Numbers

Open Library
OL19215786M
ISBN 13
9780494163634

History

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January 24, 2010 Edited by WorkBot add more information to works
December 11, 2009 Created by WorkBot add works page