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January 24, 2010 | History

The role of phosphorylation in TRA-1 regulation of sex determination in Caenorhabditis elegans 1 edition

The role of phosphorylation in TRA-1 regulation of sex determination i ...
Kathleen Dawson

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The role of phosphorylation in TRA-1 regulation of sex determination in Caenorhabditis elegans.

Published 2006 .
Written in English.

About the Book

The transcription factor TRA-1 is the global regulator of sex determination in C. elegans. Hermaphrodite development requires TRA-1 activity, and in its absence males develop. Hermaphrodites accumulate high levels of a TRA-1 phosphoisoform (Mr ≈ 90-100kD) which we believe results from proteolytic cleavage of full-length TRA-1 (Mr = 135kDa). We hypothesized that PKA phosphorylation targets TRA-1 for proteolysis, thereby producing the active form of TRA-1.To test whether PKA is involved in sex determination, I expressed a constitutively active form of PKA (PKAc*) in adult animals. Increased PKA activity did not feminize the intestine of male worms, as judged by a lack of expression of hermaphrodite-specific fluorescent reporter constructs. Deletion of a cluster of potential PKA target sites from TRA-1 revealed that residues 650-679 are required for TRA-1 phosphorylation. Unexpectedly this phosphorylation is neither necessary for the production of the truncated isoform, nor is it required for any aspect of hermaphrodite development.

Edition Notes

Source: Masters Abstracts International, Volume: 44-06, page: 2699.

Thesis (M.Sc.)--University of Toronto, 2006.

Electronic version licensed for access by U. of T. users.

ROBARTS MICROTEXT copy on microfiche.

The Physical Object

Pagination
111 leaves.
Number of pages
111

ID Numbers

Open Library
OL19215780M
ISBN 13
9780494163627

History

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January 24, 2010 Edited by WorkBot add more information to works
December 11, 2009 Created by WorkBot add works page