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Last edited by WorkBot
January 24, 2010 | History

Electrokinetically-driven micofluidic devices for DNA extraction and analysis 1 edition

Electrokinetically-driven micofluidic devices for DNA extraction and a ...
Roberto Venditti

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Electrokinetically-driven micofluidic devices for DNA extraction and analysis.

Published 2006 .
Written in English.

About the Book

This thesis focuses on electrokinetically-driven microfluidic devices for DNA analysis. These are designed, optimized via numerical simulation, and experimentally tested. Processes considered include solid phase extraction (SPE) of DNA, and DNA hybridization for the detection of single-nucleotide polymorphism (SNP) genetic mutations. Also included is an experimental analysis of the influence of temperature on the zeta potential parameter (and microchannel flow velocity). The SPE device was successful with input samples of pre-purified lambda-DNA and whole HeLa cancer cells, with a 68% maximum efficiency. The DNA hybridization device was capable of detecting the SNP associated with the childhood disease, Spinal Muscular Atrophy, using both pre-purified and polymerase chain reaction (PCR)-amplified DNA. Zeta potential experiments concluded that many buffers commonly-used in microfluidic applications experience a strong dependence on temperature, contrary to common treatment of this variable. Failure to compensate for this can result in underestimation of in-channel flow velocity as high as 30%.

Edition Notes

Source: Masters Abstracts International, Volume: 44-06, page: 2983.

Thesis (M.A.Sc.)--University of Toronto, 2006.

Electronic version licensed for access by U. of T. users.

ROBARTS MICROTEXT copy on microfiche.

The Physical Object

121 leaves.
Number of pages

ID Numbers

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History Created December 11, 2009 · 2 revisions Download catalog record: RDF / JSON

January 24, 2010 Edited by WorkBot add more information to works
December 11, 2009 Created by WorkBot add works page